Please use this identifier to cite or link to this item:
http://bura.brunel.ac.uk/handle/2438/22982
Title: | Interfacial biocatalytic performance of nanofiber-supported β-galactosidase for production of galacto-oligosaccharides |
Authors: | Misson, M Jin, B Dai, S Zhang, H |
Keywords: | β-galactosidase;polymer nanofibers;nanobiocatalyst;interfacial characterization;interfacial reaction;conformational change |
Issue Date: | 6-Jan-2020 |
Publisher: | MDPI AG |
Citation: | Misson, M., Jin, B., Dai, S. and Zhang, H. (2020) ‘Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides’, Catalysts, 10 (1), 81, pp. 1-12. doi: 10.3390/catal10010081. |
Abstract: | © 2020 by the authors. Molecular distribution, structural conformation and catalytic activity at the interface between enzyme and its immobilising support are vital in the enzymatic reactions for producing bioproducts. In this study, a nanobiocatalyst assembly, β-galactosidase immobilized on chemically modified electrospun polystyrene nanofibers (PSNF), was synthesized for converting lactose into galacto-oligosaccharides (GOS). Characterization results using scanning electron microscopy (SEM) and fluorescence analysis of fluorescein isothiocyanat (FITC) labelled β-galactosidase revealed homogenous enzyme immobilization, thin layer structural conformation and biochemical functionalities of the nanobiocatalyst assembly. The β-galactosidase/PSNF assembly displayed enhanced enzyme catalytic performance at a residence time of around 1 min in a disc-stacked column reactor. A GOS yield of 41% and a lactose conversion of 88% was achieved at the initial lactose concentration of 300 g/L at this residence time. This system provided a controllable contact time of products and substrates on the nanofiber surface and could be used for products which are sensitive to the duration of nanobiocatalysis. |
URI: | https://bura.brunel.ac.uk/handle/2438/22982 |
DOI: | https://doi.org/10.3390/catal10010081 |
Appears in Collections: | Chemistry |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
FullText.pdf | 2.03 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License