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DC Field | Value | Language |
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dc.contributor.author | Markandran, K | - |
dc.contributor.author | Xuan, JVLE | - |
dc.contributor.author | Yu, H | - |
dc.contributor.author | Shun, LM | - |
dc.contributor.author | Ferenczi, MA | - |
dc.date.accessioned | 2021-12-01T11:25:57Z | - |
dc.date.available | 2021-12-01T11:25:57Z | - |
dc.date.issued | 2021-08-19 | - |
dc.identifier | e221 | - |
dc.identifier.citation | Markandran, K., Xuan, J.V.L.E., Yu, H., Shun , L.M. and Ferenczi, M.A. (2021) 'Mn<sup>2+</sup>-Phos-Tag Polyacrylamide for the Quantification of Protein Phosphorylation Levels', Current Protocols, 2021, 1 (8), e221, pp. 1 - 32 (32). doi: 10.1002/cpz1.221. | en_US |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/23651 | - |
dc.description.abstract | This paper provides a guideline for optimizing and utilizing Mn2+ Phos-tag gel technology to separate phosphorylated proteins from their unphosphorylated counterparts. It provides key insights into methods for careful sample preparation and experimental directions for determining the appropriate Phos-tag gel compositions and electrophoresis and western blotting conditions. This protocol has been used to successfully resolve proteins extracted from cardiac and skeletal muscles. The guidelines can be extended for optimizing protocols to resolve proteins from other cells or tissue sources. With this, phosphoproteomics and the elucidation of underlying mechanisms of disease progression can be accelerated. Copyright © 2021 The Authors. | en_US |
dc.description.sponsorship | Singapore Ministry of Education under its Academic Research Fund Tier 2 (Project No. MOE2016-T2-1-106). The APC was funded by the Ministry of Education Research Funding. | - |
dc.format.extent | 1 - 32 (32) | - |
dc.format.medium | Electronic | - |
dc.language | en | - |
dc.language.iso | en_US | en_US |
dc.publisher | Wiley | en_US |
dc.rights | © 2021 The Authors. Current Protocols published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.subject | electrophoresis | en_US |
dc.subject | phosphoproteomics | en_US |
dc.subject | phosphorylation | en_US |
dc.subject | Phos-tag | en_US |
dc.subject | western blotting | en_US |
dc.title | Mn<sup>2+</sup>-Phos-Tag Polyacrylamide for the Quantification of Protein Phosphorylation Levels | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.1002/cpz1.221 | - |
dc.relation.isPartOf | Current Protocols | - |
pubs.issue | 8 | - |
pubs.publication-status | Published | - |
pubs.volume | 1 | - |
dc.identifier.eissn | 2691-1299 | - |
Appears in Collections: | Dept of Health Sciences Research Papers |
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FullText.pdf | 1.54 MB | Adobe PDF | View/Open |
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