Please use this identifier to cite or link to this item:
http://bura.brunel.ac.uk/handle/2438/24010
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wong, SSW | - |
dc.contributor.author | Dellière, S | - |
dc.contributor.author | Schiefermeier-Mach, N | - |
dc.contributor.author | Lechner, L | - |
dc.contributor.author | Perkhofer, S | - |
dc.contributor.author | Bomme, P | - |
dc.contributor.author | Fontaine, T | - |
dc.contributor.author | Schlosser, AG | - |
dc.contributor.author | Sorensen, GL | - |
dc.contributor.author | Madan, T | - |
dc.contributor.author | Kishore, U | - |
dc.contributor.author | Aimanianda, V | - |
dc.date.accessioned | 2022-01-27T17:42:59Z | - |
dc.date.available | 2022-12 | - |
dc.date.available | 2022-01-27T17:42:59Z | - |
dc.date.issued | 2022-01-10 | - |
dc.identifier | 100072 | - |
dc.identifier.citation | Wong, S.S.W., Dellière, S., Schiefermeier-Mach, N., Lechner, L., Perkhofer, S., Bomme, P., Fontaine, T., Schlosser, A.G., Sorensen, G.L., Madan, T., Kishore, U. and Aimanianda, V. (2022) 'Surfactant protein D inhibits growth, alters cell surface polysaccharide exposure and immune activation potential of Aspergillus fumigatus', The Cell Surface, 8, 100072, pp. 1-12. doi: 10.1016/j.tcsw.2022.100072. | en_US |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/24010 | - |
dc.description.abstract | © 2022 The Authors. Humoral immunity plays a defensive role against invading microbes. However, it has been largely overlooked with respect to Aspergillus fumigatus, an airborne fungal pathogen. Previously, we have demonstrated that surfactant protein D (SP-D), a major humoral component in human lung-alveoli, recognizes A. fumigatus conidial surface exposed melanin pigment. Through binding to melanin, SP-D opsonizes conidia, facilitates conidial phagocytosis, and induces the expression of protective pro-inflammatory cytokines in the phagocytic cells. In addition to melanin, SP-D also interacts with galactomannan (GM) and galactosaminogalactan (GAG), the cell wall polysaccharides exposed on germinating conidial surfaces. Therefore, we aimed at unravelling the biological significance of SP-D during the germination process. Here, we demonstrate that SP-D exerts direct fungistatic activity by restricting A. fumigatus hyphal growth. Conidial germination in the presence of SP-D significantly increased the exposure of cell wall polysaccharides chitin, α-1,3-glucan and GAG, and decreased β-1,3-glucan exposure on hyphae, but that of GM was unaltered. Hyphae grown in presence of SP-D showed positive immunolabelling for SP-D. Additionally, SP-D treated hyphae induced lower levels of pro-inflammatory cytokine, but increased IL-10 (anti-inflammatory cytokine) and IL-8 (a chemokine) secretion by human peripheral blood mononuclear cells (PBMCs), compared to control hyphae. Moreover, germ tube surface modifications due to SP-D treatment resulted in an increased hyphal susceptibility to voriconazole, an antifungal drug. It appears that SP-D exerts its anti-A. fumigatus functions via a range of mechanisms including hyphal growth-restriction, hyphal surface modification, masking of hyphal surface polysaccharides and thus altering hyphal immunostimulatory properties. | en_US |
dc.description.sponsorship | Pasteur Roux-Cantarini Fellowship; UtechS Photonic BioImaging (Imagopole), C2RT, Institut Pasteur, supported by the French National Research Agency (France BioImaging; ANR-10–INBS–04; Investments for the Future). | en_US |
dc.format.extent | 100072 - 100072 | - |
dc.format.extent | 1 - 12 | - |
dc.format.medium | Electronic | - |
dc.language | English | - |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier BV | en_US |
dc.rights | © 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/). | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.subject | Aspergillus fumigatus | en_US |
dc.subject | innate immunity | en_US |
dc.subject | pattern-recognition receptor | en_US |
dc.subject | surfactant protein D | en_US |
dc.subject | cell wall | en_US |
dc.subject | polysaccharides | en_US |
dc.title | Surfactant protein D inhibits growth, alters cell surface polysaccharide exposure and immune activation potential of Aspergillus fumigatus | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.1016/j.tcsw.2022.100072 | - |
dc.relation.isPartOf | The Cell Surface | - |
pubs.publication-status | Published | - |
pubs.volume | 8 | - |
dc.identifier.eissn | 2468-2330 | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
FullText.pdf | 7.18 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License