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DC Field | Value | Language |
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dc.contributor.author | Trinh, AL | - |
dc.contributor.author | Esposito, A | - |
dc.date.accessioned | 2022-11-03T13:06:10Z | - |
dc.date.available | 2022-11-03T13:06:10Z | - |
dc.date.issued | 2021-06-03 | - |
dc.identifier | ORCiD ID: Andrew L. Trinh: https://orcid.org/0000-0001-6547-5494; Alessandro Esposito: https://orcid.org/0000-0002-5051-091X | - |
dc.identifier | 428070 | - |
dc.identifier.citation | Trinh, A.L. and Esposito, A. (2021) 'Biochemical resolving power of fluorescence lifetime imaging: untangling the roles of the instrument response function and photon-statistics', Biomedical Optics Express, 12 (7), 428070, pp. 3775 - 3788. doi: 10.1364/BOE.428070. | en_US |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/25427 | - |
dc.description | Data availability: Jupyter and Mathematica Notebooks underlying the results presented in this paper are freely available at the GitHub repository: A. L. Trinh and A. Esposito, “Esposito-Lab/BiochemicalResolution,” Zenodo (2021), retrieved https://doi.org/10.5281/zenodo.4483760. | en_US |
dc.description.abstract | Copyright © The Author(s) 2021. A deeper understanding of spatial resolution has led to innovations in microscopy and the disruption of biomedical research, as with super-resolution microscopy. To foster similar advances in time-resolved and spectral imaging, we have previously introduced the concept of ‘biochemical resolving power’ in fluorescence microscopy. Here, we apply those concepts to investigate how the instrument response function (IRF), sampling conditions, and photon-statistics limit the biochemical resolution of fluorescence lifetime microscopy. Using Fisher information analysis and Monte Carlo simulations, we reveal the complex dependencies between photon-statistics and the IRF, permitting us to quantify resolution limits that have been poorly understood (e.g., the minimum resolvable decay time for a given width of the IRF and photon-statistics) or previously underappreciated (e.g., optimization of the IRF for biochemical detection). With this work, we unravel common misunderstandings on the role of the IRF and provide theoretical insights with significant practical implications on the design and use of time-resolved instrumentation. | en_US |
dc.description.sponsorship | Cancer Research UK (OncoLive, C54674/A27487); Medical Research Council (MC_UU_12022/1, MC_UU_12022/8). | en_US |
dc.format.extent | 3775 - 3788 | - |
dc.format.medium | Electronic | - |
dc.language | English | - |
dc.language.iso | en_US | en_US |
dc.publisher | Optica Publishing Group | en_US |
dc.rights | Copyright © The Author(s) 2021. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/). Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI. | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.title | Biochemical resolving power of fluorescence lifetime imaging: untangling the roles of the instrument response function and photon-statistics | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.1364/BOE.428070 | - |
dc.relation.isPartOf | Biomedical Optics Express | - |
pubs.issue | 7 | - |
pubs.publication-status | Published | - |
pubs.volume | 12 | - |
dc.identifier.eissn | 2156-7085 | - |
dc.rights.holder | The Author(s) | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
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