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DC Field | Value | Language |
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dc.contributor.author | Proctor, CR | - |
dc.contributor.author | Maslova, E | - |
dc.contributor.author | Dzalbe, S | - |
dc.contributor.author | Rudolph, CJ | - |
dc.contributor.author | McCarthy, RR | - |
dc.date.accessioned | 2022-11-24T14:37:58Z | - |
dc.date.available | 2022-11-24T14:37:58Z | - |
dc.date.issued | 2022-11-22 | - |
dc.identifier | ORCID iDs: Rubén de Dios https://orcid.org/0000-0001-6704-9149; Christian Rudolph https://orcid.org/0000-0003-2493-3748; Ronan R McCarthy https://orcid.org/0000-0002-7480-6352. | - |
dc.identifier | e16397 | - |
dc.identifier.citation | Proctor, C.R. et. al. (2022), 'Artificial sweeteners inhibit multidrug-resistant pathogen growth and potentiate antibiotic activity' in EMBO Molecular Medicine. 15 (1), e16397, pp.1 - 21. doi: 10.15252/emmm.202216397. | en_US |
dc.identifier.issn | 1757-4676 | - |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/25534 | - |
dc.description | Disclosure and competing interests statement: Brunel University London has two patents covering the therapeutic use of artificial sweeteners and their use to potentiate antibiotic activity. | - |
dc.description | Data availability The RNA-seq datasets produced in this study (gene expression dataset series titled "Alteration of global transcription by the artifi- cial sweetener acesulfame-K in Acinetobacter baumannii AB5075") are available at the National Center for Biotechnology Information Gene Expression Omnibus public database under accession number GSE199706 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi? acc=GSE199706). | - |
dc.description.abstract | Copyright © 2022 The Authors. Antimicrobial resistance is one of the most pressing concerns of our time. The human diet is rich with compounds that alter bacterial gut communities and virulence-associated behaviours, suggesting food additives may be a niche for the discovery of novel anti-virulence compounds. Here, we identify three artificial sweeteners, saccharin, cyclamate and acesulfame-K (ace-K), that have a major growth inhibitory effect on priority pathogens. We further characterise the impact of ace-K on multidrug-resistant Acinetobacter baumannii, demonstrating that it can disable virulence behaviours such as biofilm formation, motility and the ability to acquire exogenous antibiotic-resistant genes. Further analysis revealed the mechanism of growth inhibition is through bulge-mediated cell lysis and that cells can be rescued by cation supplementation. Antibiotic sensitivity assays demonstrated that at sub-lethal concentrations, ace-K can resensitise A. baumannii to last resort antibiotics, including carbapenems. Using a novel ex vivo porcine skin wound model, we show that ace-K antimicrobial activity is maintained in the wound microenvironment. Our findings demonstrate the influence of artificial sweeteners on pathogen behaviour and uncover their therapeutic potential. | en_US |
dc.description.sponsorship | British Society for Antimicrobial Chemotherapy BSAC-2018-0095; NC3Rs PhD Studentship NC/V001582/1; Biotechnology and Biological Sciences Research Council New Investigator Award BB/V007823/1; Academy of Medical Sciences/the Wellcome Trust/the Government Department of Business, Energy and Industrial Strategy/the Bri- tish Heart Foundation/Diabetes UK Springboard Award [SBF006\1040; Research Grant BB/T007168/1 from the Biotechnology and Biological Sciences Research Council. | en_US |
dc.format.extent | 1 - 21 | - |
dc.format.medium | Print-Electronic | - |
dc.language | English | - |
dc.publisher | EMBO | en_US |
dc.rights | Copyright © 2022 The Authors. Published under the terms of the CC BY 4.0 license. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | acinetobacter baumannii | en_US |
dc.subject | antimicrobial | en_US |
dc.subject | artificial sweetener | en_US |
dc.subject | biofilm | en_US |
dc.title | Artificial sweeteners inhibit multidrug‐resistant pathogen growth and potentiate antibiotic activity | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.15252/emmm.202216397 | - |
dc.relation.isPartOf | EMBO Molecular Medicine | - |
pubs.issue | 1 | - |
pubs.publication-status | Published online | - |
pubs.volume | 15 | - |
dc.identifier.eissn | 1757-4684 | - |
dc.rights.holder | The Authors | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
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FullText.pdf | Copyright © 2022 The Authors. Published under the terms of the CC BY 4.0 license. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | 3.58 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License