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DC Field | Value | Language |
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dc.contributor.author | Box, C | - |
dc.contributor.author | Pennington, C | - |
dc.contributor.author | Hare, S | - |
dc.contributor.author | Porter, S | - |
dc.contributor.author | Edwards, D | - |
dc.contributor.author | Eccles, S | - |
dc.contributor.author | Crompton, M | - |
dc.contributor.author | Harvey, A | - |
dc.date.accessioned | 2023-06-29T12:29:57Z | - |
dc.date.available | 2023-06-29T12:29:57Z | - |
dc.date.issued | 2023-06-28 | - |
dc.identifier | ORCID iDs: Carol Box https://orcid.org/0000-0002-8919-8724; Caroline Pennington https://orcid.org/0009-0004-1068-9515; Amanda Harvey https://orcid.org/0000-0003-0257-641X. | - |
dc.identifier | 10757 | - |
dc.identifier.citation | Box, C. et al. (2023) 'Brk/PTK6 and Involucrin Expression May Predict Breast Cancer Cell Responses to Vitamin D', International Journal of Molecular Sciences, 2023, 24, 10757, pp. 1 - 14. doi: 10.3390/ijms241310757. | en_US |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/26750 | - |
dc.description | Data Availability Statement: No datasets were generated during this study | en_US |
dc.description.abstract | Copyright © 2023 by the authors. The process of human embryonic mammary development gives rise to the structures in which mammary cells share a developmental lineage with skin epithelial cells such as keratinocytes. As some breast carcinomas have previously been shown to express high levels of involucrin, a marker of keratinocyte differentiation, we hypothesised that some breast tumours may de-differentiate to a keratinocyte-derived ‘evolutionary history’. To confirm our hypothesis, we investigated the frequency of involucrin expression along with that of Brk, a tyrosine kinase expressed in up to 86% of breast carcinomas whose normal expression patterns are restricted to differentiating epithelial cells, most notably those in the skin (keratinocytes) and the gastrointestinal tract. We found that involucrin, a keratinocyte differentiation marker, was expressed in a high proportion (78%) of breast carcinoma samples and cell lines. Interestingly, tumour samples found to express high levels of involucrin were also shown to express Brk. 1,25-dihydroxyvitamin D3, a known differentiation agent and potential anti-cancer agent, decreased proliferation in the breast cancer cell lines that expressed both involucrin and Brk, whereas the Brk/involucrin negative cell lines tested were less susceptible. In addition, responses to 1,25-dihydroxyvitamin D3 were not correlated with vitamin D receptor expression. These data contribute to the growing body of evidence suggesting that cellular responses to 1,25-dihydroxyvitamin D3 are potentially independent of vitamin D receptor status and provide an insight into potential markers, such as Brk and/or involucrin that could predict therapeutic responses to 1,25-dihydroxyvitamin D3. | en_US |
dc.description.sponsorship | This work was supported primarily by project grants from the Breast Cancer Campaign (2006NovPR19 awarded to MRC/SE/AH) and the EU Framework Programme 6 Cancer Degradome project (LSHC-CT-2003-503297). The authors acknowledge NHS funding to the NIHR Biomedical Research Centre. | en_US |
dc.format.extent | 1 - 14 | - |
dc.format.medium | Electronic | - |
dc.language | English | - |
dc.language.iso | en_US | en_US |
dc.publisher | MDPI | en_US |
dc.rights | Copyright © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | 1,25-dihydroxyvitamin D3 | en_US |
dc.subject | breast cancer | en_US |
dc.subject | breast tumour kinase (Brk) | en_US |
dc.subject | protein tyrosine kinase 6 (PTK6) | en_US |
dc.subject | differentiation | en_US |
dc.subject | involucrin | en_US |
dc.title | Brk/PTK6 and Involucrin Expression May Predict Breast Cancer Cell Responses to Vitamin D | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.3390/ijms241310757 | - |
dc.relation.isPartOf | International Journal of Molecular Sciences | - |
pubs.publication-status | Published online | - |
pubs.volume | 24 | - |
dc.identifier.eissn | 1422-0067 | - |
dc.rights.holder | The authors | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
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