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DC Field | Value | Language |
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dc.contributor.author | Micou, S | - |
dc.contributor.author | Forner-Piquer, I | - |
dc.contributor.author | Cresto, N | - |
dc.contributor.author | Zassot, T | - |
dc.contributor.author | Drouard, A | - |
dc.contributor.author | Larbi, M | - |
dc.contributor.author | Mangoni, ME | - |
dc.contributor.author | Audinat, E | - |
dc.contributor.author | Jopling, C | - |
dc.contributor.author | Faucherre, A | - |
dc.contributor.author | Marchi, N | - |
dc.contributor.author | Torrente, AG | - |
dc.date.accessioned | 2023-10-03T11:13:55Z | - |
dc.date.available | 2023-10-03T11:13:55Z | - |
dc.date.issued | 2023-05-16 | - |
dc.identifier | ORCID iDs: Nicola Marchi https://orcid.org/0000-0001-9124-0226; Isabel Forner-Piquer https://orcid.org/0000-0002-5315-3858; Angelo G. Torrente https://orcid.org/0000-0003-1721-6564. | - |
dc.identifier | 103196 | - |
dc.identifier.citation | Micou, S. et al. (2023) 'High-speed optical mapping of heart and brain voltage activities in zebrafish larvae exposed to environmental contaminants', Environmental Technology and Innovation, 31, 103196, pp. 1 - 13. doi: 10.1016/j.eti.2023.103196. | en_US |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/27299 | - |
dc.description | Data availability: Data will be made available on request. | en_US |
dc.description | Supplementary data are available online at https://www.sciencedirect.com/science/article/pii/S235218642300192X?via%3Dihub#appSB . | - |
dc.description.abstract | Copyright © 2023 The Author(s). Environmental contaminants represent a poorly understood ecotoxicological and health risk. Here, we advanced a high-speed optical mapping (OM) technique to non-invasively track voltage dynamics in living zebrafish larvae’s heart and brain and investigate the effects of selected pesticides. OM allowed high resolution ( 17x) and fast acquisition (100 to 200 frames/s) of the voltage signal generated in the heart and brain after immersion of the zebrafish larvae in a voltage-sensitive dye. First, we used varying temperatures (20 °C to 25 °C) to test the adequacy of OM in capturing cardiac and brain voltage changes. Then, we tested the effects of glyphosate or a selected pesticide cocktail (2 to 120 h post-fertilization), accounting for their environmental thresholds and mimicking high-level exposure. Glyphosate (0.1 and 1000 g/L) and the pesticide cocktail (0.1 and 10 g/L) did not alter cardiac activity, except for a trend increase in heart rate variability at high glyphosate dose. Fourier transform (FT) analyses indicated that glyphosate reduced the abundance of low-amplitude voltage activities in the brain at the target low-frequency range of 0.2–15 Hz. The anatomical fragmentation of the brain into four regions, right and left diencephalon (RD and LD) and right and left optic tectum (ROT and LOT), confirmed the impact of glyphosate on the larvae brain and revealed a specific adaptation to the pesticide cocktail in the RD and ROT regions. In summary, OM captured heart and brain voltage changes in zebrafish larvae, with discrete patterns of brain depolarization in the presence of specific water contaminants. Here we discuss the relevance of these findings to ecotoxicology and exposome research. | en_US |
dc.description.sponsorship | This work was supported by ANR-Hepatobrain and Epidimicmac ANSES to NM, and “Soutien à la Recherche 2021” of the University of Montpellier and Fondation pour la Recherche sur le Cerveau, France: Espoir en tête 2022/23 to AGT. Partially funded by OptoFish ANSES, ANR-EpiCatcher, ANR/Era-Net Neu-Vasc to NM and the Fondation pour la Recherche Médicale, France (FRM, grant DPC2017 to M.E.M). | en_US |
dc.format.extent | 1 - 13 | - |
dc.format.medium | Electronic | - |
dc.language | English | - |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | Copyright © 2023 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/). | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.subject | heart rate | en_US |
dc.subject | heart depolarization | en_US |
dc.subject | brain voltage activity | en_US |
dc.subject | pesticide cocktail | en_US |
dc.subject | Glyphosate | en_US |
dc.subject | optical mapping | en_US |
dc.subject | exposome | en_US |
dc.title | High-speed optical mapping of heart and brain voltage activities in zebrafish larvae exposed to environmental contaminants | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.1016/j.eti.2023.103196 | - |
dc.relation.isPartOf | Environmental Technology and Innovation | - |
pubs.publication-status | Published | - |
pubs.volume | 31 | - |
dc.identifier.eissn | 2352-1864 | - |
dc.rights.holder | The Author(s) | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
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FullText.pdf | Copyright © 2023 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/). | 3.29 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License