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DC Field | Value | Language |
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dc.contributor.author | Andrade Godoi, FG | - |
dc.contributor.author | Forner-Piquer, I | - |
dc.contributor.author | Randazzo, B | - |
dc.contributor.author | Habibi, HR | - |
dc.contributor.author | Lo Nostro, FL | - |
dc.contributor.author | Guimarães Moreira, R | - |
dc.contributor.author | Carnevali, OI | - |
dc.date.accessioned | 2023-12-06T10:58:24Z | - |
dc.date.available | 2021-06-14 | - |
dc.date.available | 2023-12-06T10:58:24Z | - |
dc.date.issued | 2021-06-14 | - |
dc.identifier | ORCID iD: Isabel Forner-Piquer https://orcid.org/0000-0002-5315-3858 | - |
dc.identifier | 677853 | - |
dc.identifier.citation | Andrade Godoi, F.G. et al. (2021) 'Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary', Frontiers in Endocrinology, 12, 677853, pp. 1 - 10. doi: 10.3389/fendo.2021.677853. | en_US |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/27811 | - |
dc.description | Data Availability Statement: The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation. | en_US |
dc.description.abstract | Copyright © 2021 Godoi, Forner-Piquer, Randazzo, Habibi, Lo Nostro, Moreira and Carnevali. Di-isononyl phthalate (DiNP) is a plasticizer reported to elicit hormone-like activity and disrupt metabolism and reproduction in fish and other vertebrates. In general, phthalates have been used at high concentrations beyond reported environmental levels to assess their adverse effects on fish gonadal physiology. The present study exposed adult female zebrafish to a wide range of DiNP concentrations [0.42 µg L−1 (10−9 M), 4.2 µg L−1 (10−8 M), and 42 µg L−1 (10−7 M)] for 21 days. We evaluated gene expression profiles related to apoptosis, autophagy, and oxidative stress; DNA fragmentation (TUNEL assay: terminal deoxynucleotidyl transferase dUTP nick end labeling) and caspase activity (CAS3) were also examined. Exposure to 0.42 and 4.2 µg L−1 upregulated the genes coding for tnfa and baxa, sod1, prkaa1, respectively. CAS3 immunohistochemistry revealed a higher number of positive vitellogenic oocytes in ovaries exposed to 0.42 µg L−1. Subsequently, we examined the relationship between CAS3 signaling and DNA fragmentation. Accordingly, DNA fragmentation was observed in vitellogenic follicles of fish exposed to 0.42 and 4.2 μg L−1. Our results demonstrate that follicular atresia can occur after exposure to environmental levels of DiNP for 21 days, which may adversely affect the reproductive performance of female zebrafish in a non-monotonic manner. | en_US |
dc.description.sponsorship | Progetti di Rilevante Interesse Nazionale (PRIN) 2010–2011 (prot 2010W87LBJ) to OC; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP; Processes: 2018/10495-0; 2017/07139-5, 2017/11530-1 and 2014/16320-7) to FG and RM. | en_US |
dc.format.extent | 1 - 10 | - |
dc.format.medium | Electronic | - |
dc.language | English | - |
dc.language.iso | en_US | en_US |
dc.publisher | Frontiers Media | en_US |
dc.rights | Copyright © 2021 Godoi, Forner-Piquer, Randazzo, Habibi, Lo Nostro, Moreira and Carnevali. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | Danio rerio | en_US |
dc.subject | endocrine disruption | en_US |
dc.subject | phthalate | en_US |
dc.subject | aquatic toxicology | en_US |
dc.subject | reproductive biomarkers | en_US |
dc.subject | follicular atresia | en_US |
dc.title | Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.3389/fendo.2021.677853 | - |
dc.relation.isPartOf | Frontiers in Endocrinology | - |
pubs.publication-status | Published | - |
pubs.volume | 12 | - |
dc.identifier.eissn | 1664-2392 | - |
dc.rights.holder | Godoi, Forner-Piquer, Randazzo, Habibi, Lo Nostro, Moreira and Carnevali | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
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FullText.pdf | Copyright © 2021 Godoi, Forner-Piquer, Randazzo, Habibi, Lo Nostro, Moreira and Carnevali. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | 4.09 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License