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DC Field | Value | Language |
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dc.contributor.author | Larocque, G | - |
dc.contributor.author | Moore, DJ | - |
dc.contributor.author | Sittewelle, M | - |
dc.contributor.author | Kuey, C | - |
dc.contributor.author | Hetmanski, JHR | - |
dc.contributor.author | La-Borde, PJ | - |
dc.contributor.author | Wilson, BJ | - |
dc.contributor.author | Clarke, NI | - |
dc.contributor.author | Caswell, PT | - |
dc.contributor.author | Royle, SJ | - |
dc.date.accessioned | 2024-03-13T10:57:19Z | - |
dc.date.available | 2024-03-13T10:57:19Z | - |
dc.date.issued | 2021-07-21 | - |
dc.identifier | ORCiD: Gabrielle Larocque https://orcid.org/0000-0001-8295-9378 | - |
dc.identifier | ORCiD: Daniel J. Moore https://orcid.org/0000-0003-1553-0939 | - |
dc.identifier | ORCiD: Méghane Sittewelle https://orcid.org/0000-0002-9383-6653 | - |
dc.identifier | ORCiD: Cansu Kuey https://orcid.org/0000-0002-7992-3523 | - |
dc.identifier | ORCiD: Joseph H.R. Hetmanski https://orcid.org/0000-0002-1493-351X | - |
dc.identifier | ORCiD: Penelope J. La-Borde https://orcid.org/0000-0002-9499-0062 | - |
dc.identifier | ORCiD: Beverley J. Wilson https://orcid.org/0000-0002-5425-7800 | - |
dc.identifier | ORCiD: Nicholas I. Clarke https://orcid.org/0000-0003-3297-8604 | - |
dc.identifier | ORCiD: Patrick T. Caswell https://orcid.org/0000-0002-2633-2324 | - |
dc.identifier | ORCiD: Stephen J. Royle https://orcid.org/0000-0001-8927-6967 | - |
dc.identifier | e202009028 | - |
dc.identifier.citation | Larocque, G. et al. (2021) 'Intracellular nanovesicles mediate α5β1 integrin trafficking during cell migration', Journal of Cell Biology, 220 (10), e202009028, pp. 1 - 17 (+6 supplementary pp.). doi: 10.1083/jcb.202009028. | en_US |
dc.identifier.issn | 0021-9525 | - |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/28526 | - |
dc.description | Data availability: The two software packages that are described in this paper, CellMigration (Royle, 2021) and CellShape (Royle, 2020), are freely available. All code that is specific to this paper is available at https://github.com/quantixed/p054p031. | en_US |
dc.description.abstract | Membrane traffic is an important regulator of cell migration through the endocytosis and recycling of cell surface receptors such as integrin heterodimers. Intracellular nanovesicles (INVs) are transport vesicles that are involved in multiple membrane trafficking steps, including the recycling pathway. The only known marker for INVs is tumor protein D54 (TPD54/TPD52L2), a member of the TPD52-like protein family. Overexpression of TPD52-like family proteins in cancer has been linked to poor prognosis and an aggressive metastatic phenotype, which suggests cell migration may be altered under these conditions. Here, we show that TPD54 directly binds membrane and associates with INVs via a conserved positively charged motif in its C terminus. We describe how other TPD52-like proteins are also associated with INVs, and we document the Rab GTPase complement of all INVs. Depletion of TPD52-like proteins inhibits cell migration and invasion, while their overexpression boosts motility. We show that inhibition of migration is likely due to altered recycling of α5β1 integrins in INVs. | en_US |
dc.description.sponsorship | Medical Research Council, Award ID: MR/P018947/1; Fonds de Recherche du Québec; University of Warwick; Medical Research Council, Award ID: MR/N014294/1. | en_US |
dc.format.extent | 1 - 17 (+ 6 supplementary pp.) | - |
dc.format.medium | Print-Electronic | - |
dc.language | English | - |
dc.language.iso | en_US | en_US |
dc.publisher | Rockefeller University Press | en_US |
dc.rights | Copyright © 2021 Larocque et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | cancer | en_US |
dc.subject | migration | en_US |
dc.subject | motility | en_US |
dc.subject | trafficking | en_US |
dc.title | Intracellular nanovesicles mediate α5β1 integrin trafficking during cell migration | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.1083/jcb.202009028 | - |
dc.relation.isPartOf | Journal of Cell Biology | - |
pubs.issue | 10 | - |
pubs.publication-status | Published | - |
pubs.volume | 220 | - |
dc.identifier.eissn | 1540-8140 | - |
dc.rights.license | https://creativecommons.org/licenses/by/4.0/legalcode.en | - |
dc.rights.holder | Larocque et al. | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
Files in This Item:
File | Description | Size | Format | |
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FullText.pdf | Copyright © 2021 Larocque et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). | 7.22 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License