Please use this identifier to cite or link to this item:
http://bura.brunel.ac.uk/handle/2438/2862
Title: | In situ off-axis holography of marine plankton |
Authors: | Krantz, EP Watson, J Hobson, PR Lampitt, RS Rogerson, A |
Keywords: | Holography;Off-axis holography;Particle holography;Plankton |
Issue Date: | 1996 |
Publisher: | SPIE |
Citation: | Proceedings of SPIE. 2652(1996) 236-243 |
Abstract: | We present an off-axis transmission holographic technique for recording marine plankton in situ within a test tank of 36,000 ml with a pulsed laser in a 40 ns interval. The holographic plate is located in air and is therefore unaffected by aquatic conditions that may cause emulsion degradation (e.g. non-uniform swelling and surface contamination). The reference beam traverses a path in air only, and thus remains unaffected by dense concentrations of plankton. Third order aberrations, notably spherical aberration and astigmatism, are suppressed to yield an experimental resolution of 7 lp/mm (70 micrometres) with a USAF 1951 target located 600 mm in water from the observation window. Plankton particle counts examined by real image reconstruction show a strong correlation with duplicate samples examined under a microscope. |
URI: | http://bura.brunel.ac.uk/handle/2438/2862 |
ISSN: | 0-8194-2026-3 |
Appears in Collections: | Electronic and Electrical Engineering Dept of Electronic and Electrical Engineering Research Papers |
Files in This Item:
File | Description | Size | Format | |
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SPIE_2652.pdf | 371.5 kB | Adobe PDF | View/Open |
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