Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/28791
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dc.contributor.authorThemis, M-
dc.contributor.authorGarimberti, E-
dc.contributor.authorHill, MA-
dc.contributor.authorAnderson, RM-
dc.date.accessioned2024-04-17T19:31:11Z-
dc.date.available2024-04-17T19:31:11Z-
dc.date.issued2013-06-13-
dc.identifierReplaces https://bura.brunel.ac.uk/handle/2438/7974-
dc.identifierORCiD: Rhona Anderson https://orcid.org/0000-0003-2258-656X-
dc.identifier.citationThemis, M. et al. (2013) 'Reduced chromosome aberration complexity in normal human bronchial epithelial cells exposed to low-LET γ-rays and high-LET α-particles', International Journal of Radiation Biology, 89 (11), pp. 934 - 943. doi: 10.3109/09553002.2013.805889.en_US
dc.identifier.issn0955-3002-
dc.identifier.urihttps://bura.brunel.ac.uk/handle/2438/28791-
dc.descriptionSupplemental material is available online at: https://www.tandfonline.com/doi/full/10.3109/09553002.2013.805889#supplemental-material-section .en_US
dc.description.abstractPurpose Cells of the lung are at risk from exposure to low and moderate doses of ionising radiation from a range of environmental and medical sources. To help assess human health risks from such exposures, a better understanding of the frequency and types of chromosome aberration initially-induced in human lung cell types is required to link initial DNA damage and rearrangements with transmission potential and, to assess how this varies with radiation quality. Materials and Methods We exposed normal human bronchial lung epithelial (NHBE) cells in vitro to 0.5 and 1 Gy low-linear energy transfer (LET) γ-rays and a low fluence of high-LET α-particles and assayed for chromosome aberrations in premature chromosome condensation (PCC) spreads by 24-colour multiplex-fluorescence in situ hybridisation (M-FISH). Results Both simple and complex aberrations were induced in a LET and dose dependent manner however, the frequency and complexity observed were reduced in comparison to that previously reported in spherical cell types after exposure to comparable doses or fluence of radiation. Approximately 1-2% of all exposed cells were categorised as being capable of transmitting radiation-induced chromosomal damage to future NHBE cell generations, irrespective of dose. Conclusion One possible mechanistic explanation for this reduced complexity is the differing geometric organisation of chromosome territories within ellipsoid nuclei compared to spherical nuclei. This study highlights the need to better understand the role of nuclear organisation in the formation of exchange aberrations and, the influence three-dimensional (3D) tissue architecture may have on this in vivo.en_US
dc.description.sponsorshipDepartment of Health, UK (Contract RRX115).en_US
dc.format.extent934 - 943-
dc.format.mediumPrint-Electronic-
dc.languageEnglish-
dc.language.isoenen_US
dc.publisherInforma Healthcare (Taylor and Francis Group)en_US
dc.rightsCopyright © 2013 The Author(s). This article has been made available through the Brunel Open Access Publishing Fund under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) and is available to view at: https://doi.org/10.3109/09553002.2013.805889-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.subjectM-FISHen_US
dc.subjectgeometry of cell nucleusen_US
dc.subjectradiation exposureen_US
dc.subjecthuman lungen_US
dc.titleReduced chromosome aberration complexity in normal human bronchial epithelial cells exposed to low-LET γ-rays and high-LET α-particlesen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.3109/09553002.2013.805889-
dc.relation.isPartOfInternational Journal of Radiation Biology-
pubs.issue11-
pubs.publication-statusPublished-
pubs.volume89-
dc.identifier.eissn1362-3095-
dc.rights.licensehttps://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.en-
dc.rights.holderThe Author(s)-
Appears in Collections:Brunel OA Publishing Fund
Dept of Life Sciences Research Papers

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