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Title: | Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism |
Authors: | Belda, E Voland, L Tremaroli, V Falony, G Adriouch, S Assmann, KE Prifti, E Aron-Wisnewsky, J Debédat, J Roy, TL Nielsen, T Amouyal, C André, S Andreelli, F Blüher, M Chakaroun, R Chilloux, J Coelho, LP Dao, MC Das, P Fellahi, S Forslund, S Galleron, N Hansen, TH Holmes, B Ji, B Pedersen, HK Le, P Chatelier, EL Lewinter, C Manneras-Holm, L Marquet, F Myridakis, A Pelloux, V Pons, N Quinquis, B Rouault, C Roume, H Salem, JE Sokolovska, N Søndertoft, NB Touch, S Vieira-Silva, S Galan, P Holst, J Gøtze, JP Køber, L Vestergaard, H Hansen, T Hercberg, S Oppert, JM Nielsen, J Letunic, I Dumas, ME Stumvoll, M Pedersen, OB Bork, P Ehrlich, SD Zucker, JD Bäckhed, F Raes, J Clément, K |
Issue Date: | 11-Jan-2022 |
Publisher: | BMJ Publishing Group on behalf of British Society of Gastroenterology |
Citation: | Belda, E. et al. (2023) 'Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism', Gut, 71 (12), pp. 2463 - 2480. doi: 10.1136/gutjnl-2021-325753. |
Abstract: | Objectives: Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear. We examined the human gut microbiome’s functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort. We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation. Design: We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice. Results: Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes. We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue. In mice, the absence or depletion of gut microbiota by antibiotics confirmed the microbial contribution to host biotin levels. Bariatric surgery, which improves metabolism and inflammation, associates with increased bacterial biotin producers and improved host systemic biotin in humans and mice. Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration. Conclusion: Strategies combining biotin and prebiotic supplementation could help prevent the deterioration of metabolic states in severe obesity. Trial registration number: NCT02059538. |
Description: | Data availability statement:
Data are available in a public, open access repository. Data are available on reasonable request. metacardis https://www.ebi.ac.uk/ena/browser/view/PRJEB41311, https://www.ebi.ac.uk/ena/browser/view/PRJEB38742, https://www.ebi.ac.uk/ena/browser/view/PRJEB37249, mouse experiments; 16S data, https://www.ebi.ac.uk/ena/browser/view/PRJEB42967, mouse experiments; nanopore data, https://www.ebi.ac.uk/ena/browser/view/PRJEB42966, Code paper analyses, https://git.ummisco.fr/ebelda/metatransitionbiotingut.git. Any methods, additional references, Nature Research reporting summaries, source data, extended data, supplementary information, acknowledgements, peer review information; details of author contributions and competing interests; and statements of data and code availability are available at https://doi.org/10.1038/ s41591-022-01688-4. . Supplementary data are included with the article online at: https://gut.bmj.com/content/gutjnl/71/12/2463.full.pdf?with-ds=yes . |
URI: | https://bura.brunel.ac.uk/handle/2438/28988 |
DOI: | https://doi.org/10.1136/gutjnl-2021-325753 |
ISSN: | 0017-5749 |
Other Identifiers: | ORCiD: Gwen Falony https://orcid.org/0000-0003-2450-0782 ORCiD: Jean Debédat https://orcid.org/0000-0002-8377-5965 ORCID: Tiphaine Le Roy https://orcid.org/0000-0002-0874-1490 ORCiD: Trine Nielsen https://orcid.org/0000-0002-2066-7895 ORCiD: Rima Chakaroun https://orcid.org/0000-0001-9901-1815 ORCiD: Antonis Myridakis https://orcid.org/0000-0003-1690-6651 ORCiD: Torben Hansen https://orcid.org/0000-0001-8748-3831 ORCiD: Fredrik Bäckhed https://orcid.org/0000-0002-4871-8818 ORCiD: Karine Clément https://orcid.org/0000-0002-2489-3355 |
Appears in Collections: | Dept of Life Sciences Research Papers |
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