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Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/29175
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dc.contributor.authorEfthymiou, A-
dc.contributor.authorMureanu, N-
dc.contributor.authorPemberton, R-
dc.contributor.authorTai-MacArthur, S-
dc.contributor.authorMastronicola, D-
dc.contributor.authorScottà, C-
dc.contributor.authorLombardi, G-
dc.contributor.authorNicolaides, KH-
dc.contributor.authorShangaris, P-
dc.date.accessioned2024-06-14T07:49:16Z-
dc.date.available2024-06-14T07:49:16Z-
dc.date.issued2022-02-25-
dc.identifierORCiD: Cristiano Scottà https://orcid.org/0000-0003-3942-5201-
dc.identifierORCiD: Panicos Shangaris https://orcid.org/0000-0003-2750-8405-
dc.identifier101204-
dc.identifier.citationEfthymiou, A. et al. (2022) 'Isolation and freezing of human peripheral blood mononuclear cells from pregnant patients', STAR Protocols, 3 (1), 101204, pp. 1 - 8. doi: 10.1016/j.xpro.2022.101204.en_US
dc.identifier.urihttps://bura.brunel.ac.uk/handle/2438/29175-
dc.descriptionData and code availability: The published article includes all datasets generated or analyzed during this study.en_US
dc.description.abstractTo analyze immune cell populations accurately, a large number of Peripheral Blood Mononuclear Cells (PBMCs) must be obtained from blood samples. Traditional manual isolation and SepMateTM isolation of PBMCs consistently yield blood-stained plasma layers and overall low numbers of CD4+ and CD8+ cells. Here, we describe an optimized protocol, using PBS with EDTA to increase PBMC yield from pregnant patients. This protocol enables analysis of CD4+, CD8+, and Regulatory T Cells and is potentially applicable to any immune cell population. For complete details on the use and execution of this protocol, please refer to the SepMateTM website https://www.stemcell.com/products/brands/SepMateTM-pbmc-isolation.html.en_US
dc.description.sponsorshipFetal Medicine Foundation, United Kingdom; National Institute for Health Research (NIHR) Biomedical Research Centre based at Guy’s and St Thomas’ NHS Foundation Trust and King’s College London and/or the NIHR Clinical Research Facility.en_US
dc.format.extent1 - 8-
dc.format.mediumElectronic-
dc.language.isoen_USen_US
dc.publisherCell Press (Elsevier)en_US
dc.rightsCopyright © 2022 The Authors. Published by Cell Press. This is an open access article under a Creative Commons license (https://creativecommons.org/licenses/by/4.0/).-
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/-
dc.subjectcell biologyen_US
dc.subjectcell isolationen_US
dc.subjecthealth sciencesen_US
dc.subjectclinical protocolen_US
dc.subjectimmunologyen_US
dc.subjectstem cellsen_US
dc.titleIsolation and freezing of human peripheral blood mononuclear cells from pregnant patientsen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1016/j.xpro.2022.101204-
dc.relation.isPartOfSTAR Protocols-
pubs.issue1-
pubs.publication-statusPublished-
pubs.volume3-
dc.identifier.eissn2666-1667-
dc.rights.licensehttps://creativecommons.org/licenses/by/4.0/lealcode.en-
dc.rights.holderThe Authors-
Appears in Collections:Dept of Life Sciences Research Papers

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