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DC Field | Value | Language |
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dc.contributor.author | Altunöz, D | - |
dc.contributor.author | Sayi Yazgan, A | - |
dc.date.accessioned | 2024-07-29T19:39:25Z | - |
dc.date.available | 2024-07-29T19:39:25Z | - |
dc.date.issued | 2021-04-10 | - |
dc.identifier | ORCiD: Doğuş Altunöz https://orcid.org/0000-0002-7799-4512 | - |
dc.identifier | ORCiD: Ayça Sayi Yazgan https://orcid.org/0000-0002-9015-8244 | - |
dc.identifier | 10 | - |
dc.identifier.citation | Altunöz, D. and Sayi Yazgan, A. (2021) 'Helicobacter-stimulated il-10-producing b cells suppress differentiation of lipopolysaccharide/helicobacter felis-activated stimulatory dendritic cells', Turkish Journal of Biology, 45 (2), pp. 214 - 224. doi: 10.3906/biy-2012-11. | en_US |
dc.identifier.issn | 1300-0152 | - |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/29463 | - |
dc.description.abstract | Regulatory B cells (Bregs) produce antiinflammatory cytokines and inhibits proinflammatory response. Recently, immunosuppressive roles of Bregs in the effector functions of dendritic cells (DCs) were demonstrated. However, cross talk between Bregs and DCs in Helicobacter infection remains unknown. Here, we showed that direct stimulation of bone marrow-derived DCs (BMDCs) with Helicobacter felis (H. felis) antigen upregulates their CD86 surface expression and causes the production of interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), interleukin-12 (IL-12), and interleukin-10 (IL-10). Furthermore, prestimulation of DCs with supernatants derived from both Helicobacter-stimulated IL-10- B (Hfstim-IL-10- B) or IL-10+ B (Hfstim-IL-10+) cells suppresses the secretion of TNF-α and IL-6, but does not affect the expression of CD86 and secretion of IL-12 by lipopolysaccharide (LPS) or H. felisactivated BM-DCs. Remarkably, soluble factors secreted by Hfstim-IL-10- B cells, but not by Hfstim-IL-10+ B cells, suppress the secretion of IL-10 by BM-DCs upon subsequent LPS stimulation. In contrast, prestimulation with BM-DCs with supernatants of Hfstim-IL-10+ B cells before H. felis antigen stimulation induces significantly their IL-10 production. Collectively, our data indicated that prestimulation with soluble factors secreted by Hfstim-IL-10+ B cells, DCs exhibit a tolerogenic phenotype in response to LPS or Helicobacter antigen by secreting high levels of IL-10, but decreased levels of IL-6 and TNF-α. | en_US |
dc.description.sponsorship | The Scientific and Technological Research Council of Turkey (TÜBİTAK) supported this study with a project number 113S174. | en_US |
dc.format.extent | 214 - 224 | - |
dc.format.medium | Print-Electronic | - |
dc.language | English | - |
dc.language.iso | en_US | en_US |
dc.publisher | TÜBİTAK Academic Journals | en_US |
dc.subject | Helicobacter felis | en_US |
dc.subject | regulatory B cells | en_US |
dc.subject | dendritic cells | en_US |
dc.subject | lipopolysaccharide | en_US |
dc.title | Helicobacter-stimulated il-10-producing b cells suppress differentiation of lipopolysaccharide/helicobacter felis-activated stimulatory dendritic cells | en_US |
dc.type | Article | en_US |
dc.date.dateAccepted | 2021-03-24 | - |
dc.identifier.doi | https://doi.org/10.3906/biy-2012-11 | - |
dc.relation.isPartOf | Turkish Journal of Biology | - |
pubs.issue | 2 | - |
pubs.publication-status | Published | - |
pubs.volume | 45 | - |
dc.identifier.eissn | 1303-6092 | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
Files in This Item:
File | Description | Size | Format | |
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Helicobacter-stimulated IL-10-producing B cells suppress differentiation of lipopolysaccharideHelicobacter felis-activated s.pdf | 1.02 MB | Adobe PDF | View/Open |
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