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DC Field | Value | Language |
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dc.contributor.author | Cerutti, C | - |
dc.contributor.author | Soblechero-Martin, P | - |
dc.contributor.author | Wu, D | - |
dc.contributor.author | Lopez-Ramirez, MA | - |
dc.contributor.author | de Vries, H | - |
dc.contributor.author | Sharrack, B | - |
dc.contributor.author | Male, DK | - |
dc.contributor.author | Romero, IA | - |
dc.date.accessioned | 2024-08-11T08:56:42Z | - |
dc.date.available | 2024-08-11T08:56:42Z | - |
dc.date.issued | 2016-05-31 | - |
dc.identifier | ORCiD: Camilla Cerutti https://orcid.org/0000-0001-9426-686X | - |
dc.identifier | 8 | - |
dc.identifier.citation | Cerutti, C. et al. (2016) 'MicroRNA-155 contributes to shear-resistant leukocyte adhesion to human brain endothelium in vitro', Fluids and Barriers of the CNS, 2016, 13 (1), 8, pp. 1 - 7. doi: 10.1186/s12987-016-0032-3. | en_US |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/29530 | - |
dc.description | Additional files are available online at: https://fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0032-3#Sec13 . | en_US |
dc.description.abstract | Background: Increased leukocyte adhesion to brain endothelial cells forming the blood-brain barrier (BBB) es sprecedes extravasation into the central nervous system (CNS) in neuroinflammatory diseasuch as multiple sclerosis (MS). Previously, we reported that microRNA-155 (miR-155) is up-regulated in MS and by inflammatory cytokines in human brain endothelium, with consequent modulation of endothelial paracellular permeability. Here, we investigated the role of endothelial miR-155 in leukocyte adhesion to the human cerebral microvascular endothelial cell line, hCMEC/D3, under shear forces mimicking blood flow in vivo. Results: Using a gain- and loss-of-function approach, we show that miR-155 up-regulation increases leukocyte firm adhesion of both monocyte and T cells to hCMEC/D3 cells. Inhibition of endogenous endothelial miR-155 reduced monocytic and T cell firm adhesion to naïve and cytokines-induced human brain endothelium. Furthermore, this effect is partially associated with modulation of the endothelial cell adhesion molecules VCAM1 and ICAM1 by miR-155. Conclusions: Our results suggest that endothelial miR-155 contribute to the regulation of leukocyte adhesion at the inflamed BBB. Taken together with previous observations, brain endothelial miR-155 may constitute a potential molecular target for treatment of neuroinflammation diseases. | en_US |
dc.description.sponsorship | This work was founded by the Multiple Sclerosis Society of Great Britain and Northern Ireland and BBSRC. | en_US |
dc.format.extent | 1 - 7 | - |
dc.format.medium | Electronic | - |
dc.language.iso | en_US | en_US |
dc.publisher | BioMed Central (part of Springer Nature) | en_US |
dc.rights | Copyright .© 2016 The Author(s). Rights and permissions: Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (https://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | blood–brain barrier | en_US |
dc.subject | cell adhesion molecules | en_US |
dc.subject | flow shear stress | en_US |
dc.subject | leukocyte adhesion | en_US |
dc.subject | microRNA‑155 | en_US |
dc.subject | neuroinflammation | en_US |
dc.title | MicroRNA-155 contributes to shear-resistant leukocyte adhesion to human brain endothelium in vitro | en_US |
dc.type | Article | en_US |
dc.date.dateAccepted | 2016-05-13 | - |
dc.identifier.doi | https://doi.org/10.1186/s12987-016-0032-3 | - |
dc.relation.isPartOf | Fluids and Barriers of the CNS | - |
pubs.issue | 1 | - |
pubs.publication-status | Published | - |
pubs.volume | 13 | - |
dc.identifier.eissn | 2045-8118 | - |
dc.rights.license | https://creativecommons.org/licenses/by/4.0/legalcode.en | - |
dc.rights.holder | The Author(s) | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
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FullText.pdf | Copyright .© 2016 The Author(s). Rights and permissions: Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (https://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | 1.89 MB | Adobe PDF | View/Open |
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