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DC Field | Value | Language |
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dc.contributor.author | Bryce, SD | - |
dc.contributor.author | Morrison, V | - |
dc.contributor.author | Craig, NJ | - |
dc.contributor.author | Forsyth, NR | - |
dc.contributor.author | Fitzsimmons, SA | - |
dc.contributor.author | Ireland, H | - |
dc.contributor.author | Cuthbert, AP | - |
dc.contributor.author | Newbold, RF | - |
dc.contributor.author | Parkinson, EK | - |
dc.coverage.spatial | 7 | en |
dc.date.accessioned | 2009-03-05T16:42:20Z | - |
dc.date.available | 2009-03-05T16:42:20Z | - |
dc.date.issued | 2002 | - |
dc.identifier.citation | Neoplasia. 4 (6) 544-550 | en |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/3081 | - |
dc.description.abstract | Human chromosome 4 was previously shown to elicit features of senescence when introduced into cell lines that map to complementation group B for senescence, including HeLa cells. Subsequently, a DNA segment encoding the pseudogene Mortality Factor 4 (MORF4) was shown to reproduce some of the effects of the intact chromosome 4 and was suggested to be a candidate mortality gene. We have identified multiple MORF4 alleles in several cell lines and tissues by sequencing and have failed to detect any cancer-specific mutations in three of the complementation group B lines (HeLa, T98G, and J82). Furthermore, MORF4 was heterozygous in these lines. These results question whether MORF4 is the chromosome 4 mortality gene. To map other candidate mortality gene(s) on this chromosome, we employed microcell-mediated monochromosome transfer to introduce either a complete copy, or defined fragments of the chromosome into HeLa cells. The introduced chromosome 4 fragments mapped the mortality gene to a region between the centromere and the marker D4S2975 (4q27), thus excluding MORF4, which maps to 4q33-q34.1. Analysis of microsatellite markers on the introduced chromosome in 59 immortal segregants identified a frequently deleted region, spanning the markers BIR0110 and D4S1557. This defines a new candidate interval of 130 kb at 4q22-q23 | en |
dc.format.extent | 204 bytes | - |
dc.format.mimetype | text/plain | - |
dc.language.iso | en | - |
dc.publisher | Neoplasia Press | en |
dc.title | A mortality gene(s) for the human adenocarcinoma line HeLa maps to a 130-kb region of human chromosome 4q22-q23 | en |
dc.type | Research Paper | en |
Appears in Collections: | Biological Sciences Dept of Life Sciences Research Papers |
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File | Description | Size | Format | |
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Embargoed Paper.txt | 204 B | Text | View/Open |
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