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Title: | The electrophoresis of transferrins in urea/polyacrylamide gels |
Authors: | Evans, RW Williams, J |
Issue Date: | 1980 |
Publisher: | Portland Press |
Citation: | Biochemical Journal. 189: 541-546 |
Abstract: | The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss in iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin and hen ovotransferrin the N-terminal and C-terminal domains of the iron-free protein were found to denature at different urea concentrations. |
URI: | http://bura.brunel.ac.uk/handle/2438/4340 |
ISSN: | 0264-6021 |
Appears in Collections: | Biological Sciences Dept of Life Sciences Research Papers |
Files in This Item:
File | Description | Size | Format | |
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biochemj-1980.pdf | 1.39 MB | Adobe PDF | View/Open |
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