Please use this identifier to cite or link to this item:
http://bura.brunel.ac.uk/handle/2438/5677
Title: | Persistent antigenic stimulation alters the transcription program in T cells, resulting in antigen-specific tolerance. |
Authors: | Anderson, PO Manzo, BA Sundstedt, A Minaee, S Symonds, A Khalid, S Rodriguez-Cabezas, ME Nicolson, K Li, S Wraith, DC Wang, P |
Keywords: | Animals;Cell cycle;Cell nucleus;Cyclin-dependent kinase inhibitor p21;Cyclin-dependent kinase inhibitor p27;Early growth response protein 2;Epitopes, T-Lymphocyte;Gene expression profiling;Gene expression regulation;Immune tolerance;Interleukin-2;Lymphocyte activation;Mice;Mice, transgenic;Oligonucleotide array sequence analysis;RNA;Reverse transcriptase polymerase chain reaction;Signal transduction;T-box domain proteins;T-Lymphocytes, Regulatory;Transcription factors;Transcription, Genetic;Transfection |
Issue Date: | 2006 |
Publisher: | Wiley-Blackwell |
Citation: | European Journal of Immunology 36(6): 1374 - 1385, Jun 2006 |
Abstract: | Repetitive antigen stimulation induces peripheral T cell tolerance in vivo. It is not known, however, whether multiple stimulations merely suppress T cell activation or, alternatively, change the transcriptional program to a distinct, tolerant state. In this study, we have discovered that STAT3 and STAT5 were activated in response to antigen stimulation in vivo, in marked contrast to the suppression of AP-1, NF-kappaB and NFAT. In addition, a number of transcription factors were induced in tolerant T cells following antigen challenge in vivo, including T-bet, Irf-1 and Egr-2. The altered transcription program in tolerant cells associates closely with the suppression of cell cycle progression and IL-2 production, as well as with the induction of IL-10. Studies of T-bet and Egr-2 show that the function of T-bet in peptide treatment-induced regulatory T cells is not associated with Th1 differentiation, but correlates with the suppression of IL-2, whereas expression of Egr-2 led to an up-regulation of the cell cycle inhibitors p21(cip1) and p27(kip). Our results demonstrate a balanced transcription program regulated by different transcription factors for T cell activation and/or tolerance during antigen-induced T cell responses. Persistent antigen stimulation can induce T cell tolerance by changing the balance of transcription factors. |
Description: | This article has been made available through the Brunel Open Access Publishing Fund and is available from the specified link - Copyright @ 2006 Wiley-Blackwell. |
URI: | http://bura.brunel.ac.uk/handle/2438/5677 |
DOI: | http://dx.doi.org/10.1002/eji.200635883 |
ISSN: | 0014-2980 |
Appears in Collections: | Biological Sciences Publications Dept of Life Sciences Research Papers |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Notice.pdf | 22.3 kB | Adobe PDF | View/Open |
Items in BURA are protected by copyright, with all rights reserved, unless otherwise indicated.