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Title: | Functional mammalian spliceosomal complex E contains SMN complex proteins in addition to U1 and U2 snRNPs |
Authors: | Makarov, EM Owen, N Bottrill, A Makarova, OV |
Keywords: | Primary gene transcripts;Mammals;mRNA;snRNP;ATP;MINX |
Issue Date: | 2012 |
Publisher: | Oxford University Press |
Citation: | Nucleic Acids Research, 40(6), 2639-2652, Mar 2012 |
Abstract: | Spliceosomes remove introns from primary gene transcripts. They assemble de novo on each intron through a series of steps that involve the incorporation of five snRNP particles and multiple non-snRNP proteins. In mammals, all the intermediate complexes have been characterized on one transcript (MINX), with the exception of the very first, complex E. We have purified this complex by two independent procedures using antibodies to either U1-A or PRPF40A proteins, which are known to associate at an early stage of assembly. We demonstrate that the purified complexes are functional in splicing using commitment assays. These complexes contain components expected to be in the E complex and a number of previously unrecognized factors, including survival of motor neurons (SMN) and proteins of the SMN-associated complex. Depletion of the SMN complex proteins from nuclear extracts inhibits formation of the E complex and causes non-productive complexes to accumulate. This suggests that the SMN complex stabilizes the association of U1 and U2 snRNPs with pre-mRNA. In addition, the antibody to PRPF40A precipitated U2 snRNPs from nuclear extracts, indicating that PRPF40A associates with U2 snRNPs. |
Description: | Copyright @ 2011 The Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
URI: | http://bura.brunel.ac.uk/handle/2438/6339 |
DOI: | http://dx.doi.org/10.1093/nar/gkr1056 |
ISSN: | 1362-4962 |
Appears in Collections: | Biological Sciences Publications Dept of Life Sciences Research Papers |
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