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Title: Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery
Authors: Takagi, M
Sanchez-Pulido, L
Petfalski, E
Vargiu, G
Samejima, K
Imamoto, N
Ponting, CP
Tollervey, D
Earnshaw, WC
Vagnarelli, P
Keywords: Chromosomes;Mitosis;Nucleolus;Phosphatases
Issue Date: 2014
Publisher: eLife Sciences Publications Ltd
Citation: Elife, 3: e01641 22 pp, May 2014
Abstract: When the nucleolus disassembles during open mitosis, many nucleolar proteins and RNAs associate with chromosomes, establishing a perichromosomal compartment coating the chromosome periphery. At present nothing is known about the function of this poorly characterised compartment. In this study, we report that the nucleolar protein Ki-67 is required for the assembly of the perichromosomal compartment in human cells. Ki-67 is a cell-cycle regulated protein phosphatase 1-binding protein that is involved in phospho-regulation of the nucleolar protein B23/nucleophosmin. Following siRNA depletion of Ki-67, NIFK, B23, nucleolin, and four novel chromosome periphery proteins all fail to associate with the periphery of human chromosomes. Correlative light and electron microscopy (CLEM) images suggest a near-complete loss of the entire perichromosomal compartment. Mitotic chromosome condensation and intrinsic structure appear normal in the absence of the perichromosomal compartment but significant differences in nucleolar reassembly and nuclear organisation are observed in post-mitotic cells.
Description: Copyright @ 2014 Booth et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Appears in Collections:Biological Sciences
Dept of Life Sciences Research Papers

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