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http://bura.brunel.ac.uk/handle/2438/8735
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DC Field | Value | Language |
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dc.contributor.author | Upton, AL | - |
dc.contributor.author | Stockum, A | - |
dc.contributor.author | Nieduszynski, CA | - |
dc.contributor.author | Lloyd, RG | - |
dc.date.accessioned | 2014-07-21T13:54:33Z | - |
dc.date.available | 2014-07-21T13:54:33Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Nature, 500(7464), 608 - 611, 2013 | en_US |
dc.identifier.issn | 1476-4687 | - |
dc.identifier.uri | http://www.nature.com/nature/journal/v500/n7464/full/nature12312.html | en |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/8735 | - |
dc.description | This is the author's accepted manuscript. The final published article is available from the link below. Copyright @ 2013 Macmillan Publishers Limited. | en_US |
dc.description.abstract | Chromosome duplication normally initiates through the assembly of replication fork complexes at defined origins1, 2. DNA synthesis by any one fork is thought to cease when it meets another travelling in the opposite direction, at which stage the replication machinery may simply dissociate before the nascent strands are finally ligated. But what actually happens is not clear. Here we present evidence consistent with the idea that every fork collision has the potential to threaten genomic integrity. In Escherichia coli this threat is kept at bay by RecG DNA translocase3 and by single-strand DNA exonucleases. Without RecG, replication initiates where forks meet through a replisome assembly mechanism normally associated with fork repair, replication restart and recombination4, 5, establishing new forks with the potential to sustain cell growth and division without an active origin. This potential is realized when roadblocks to fork progression are reduced or eliminated. It relies on the chromosome being circular, reinforcing the idea that replication initiation is triggered repeatedly by fork collision. The results reported raise the question of whether replication fork collisions have pathogenic potential for organisms that exploit several origins to replicate each chromosome. | en_US |
dc.description.sponsorship | THe MRC, the Leverhulme Trust, and the BBSRC. | en_US |
dc.language | eng | - |
dc.language.iso | en | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.subject | Chromosomes | en_US |
dc.subject | Genomic instability | en_US |
dc.subject | DNA synthesis | en_US |
dc.subject | Origin firing | en_US |
dc.title | Avoiding chromosome pathology when replication forks collide | en_US |
dc.type | Article | en_US |
dc.identifier.doi | http://dx.doi.org/10.1038/nature12312 | - |
pubs.organisational-data | /Brunel | - |
pubs.organisational-data | /Brunel/Brunel Staff by College/Department/Division | - |
pubs.organisational-data | /Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences | - |
pubs.organisational-data | /Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences/Dept of Life Sciences | - |
pubs.organisational-data | /Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences/Dept of Life Sciences/Biological Sciences | - |
pubs.organisational-data | /Brunel/Brunel Staff by Institute/Theme | - |
pubs.organisational-data | /Brunel/Brunel Staff by Institute/Theme/Institute of Environmental, Health and Societies | - |
pubs.organisational-data | /Brunel/Brunel Staff by Institute/Theme/Institute of Environmental, Health and Societies/Synthetic Biology | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Brunel Institute for Ageing Studies | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Centre for Systems and Synthetic Biology | - |
Appears in Collections: | Biological Sciences Dept of Life Sciences Research Papers |
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Fulltext.pdf | 2.18 MB | Adobe PDF | View/Open |
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