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DC Field | Value | Language |
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dc.contributor.author | Brault, J-B | - |
dc.contributor.author | Kwok, K | - |
dc.contributor.author | Li, MY | - |
dc.contributor.author | Pardigon, N | - |
dc.contributor.author | Peiris, JSM | - |
dc.contributor.author | Bruzzone, R | - |
dc.contributor.author | Despres, P | - |
dc.contributor.author | Nal, B | - |
dc.contributor.author | Wang, PG | - |
dc.date.accessioned | 2014-08-04T11:34:08Z | - |
dc.date.available | 2014-08-04T11:34:08Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | The Journal of Biological Chemistry, 287, 767-777, 2012 | en_US |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://www.jbc.org/content/287/1/767 | en |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/8820 | - |
dc.description | This article is available open access through the publisher’s website. | en_US |
dc.description.abstract | Identification and characterization of virus-host interactions are very important steps toward a better understanding of the molecular mechanisms responsible for disease progression and pathogenesis. To date, very few cellular factors involved in the life cycle of flaviviruses, which are important human pathogens, have been described. In this study, we demonstrate a crucial role for class II Arf proteins (Arf4 and Arf5) in the dengue flavivirus life cycle. We show that simultaneous depletion of Arf4 and Arf5 blocks recombinant subviral particle secretion for all four dengue serotypes. Immunostaining analysis suggests that class II Arf proteins are required at an early pre-Golgi step for dengue virus secretion. Using a horseradish peroxidase protein fused to a signal peptide, we show that class II Arfs act specifically on dengue virus secretion without altering the secretion of proteins through the constitutive secretory pathway. Co-immunoprecipitation data demonstrate that the dengue prM glycoprotein interacts with class II Arf proteins but not through its C-terminal VXPX motif. Finally, experiments performed with replication-competent dengue and yellow fever viruses demonstrate that the depletion of class II Arfs inhibits virus secretion, thus confirming their implication in the virus life cycle, although data obtained with West Nile virus pointed out the differences in virus-host interactions among flaviviruses. Our findings shed new light on a molecular mechanism used by dengue viruses during the late stages of the life cycle and demonstrate a novel function for class II Arf proteins. | en_US |
dc.description.sponsorship | Research Fund for Control of Infectious Diseases of Hong Kong and BNP Paribas Corporate and Investment Banking. | en_US |
dc.language | English | - |
dc.language.iso | en | en_US |
dc.publisher | American Society for Biochemistry and Molecular Biology | en_US |
dc.subject | ARF | en_US |
dc.subject | Flaviviruses | en_US |
dc.subject | Host-pathogen interactions | en_US |
dc.subject | Positive-strand RNA viruses | en_US |
dc.subject | Virus assembly | en_US |
dc.subject | Arf4 | en_US |
dc.subject | Arf5 | en_US |
dc.subject | Dengue virus | en_US |
dc.subject | Recombinant subviral particles | en_US |
dc.subject | Secretion | en_US |
dc.title | Class II ADP-ribosylation factors are required for efficient secretion of Dengue viruses | en_US |
dc.type | Article | en_US |
dc.identifier.doi | http://dx.doi.org/10.1074/jbc.M111.270579 | - |
pubs.organisational-data | /Brunel | - |
pubs.organisational-data | /Brunel/Brunel Staff by College/Department/Division | - |
pubs.organisational-data | /Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences | - |
pubs.organisational-data | /Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences/Dept of Life Sciences | - |
pubs.organisational-data | /Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences/Dept of Life Sciences/Biological Sciences | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Brunel Institute for Ageing Studies | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Brunel Institute of Cancer Genetics and Pharmacogenomics | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Centre for Systems and Synthetic Biology | - |
Appears in Collections: | Biological Sciences Dept of Life Sciences Research Papers |
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