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DC Field | Value | Language |
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dc.contributor.author | Cooley, C | - |
dc.contributor.author | Baird, KM | - |
dc.contributor.author | Faure, V | - |
dc.contributor.author | Wenner, T | - |
dc.contributor.author | Stewart, JL | - |
dc.contributor.author | Modino, S | - |
dc.contributor.author | Slijepcevic, P | - |
dc.contributor.author | Farr, CJ | - |
dc.contributor.author | Morrison, CG | - |
dc.date.accessioned | 2014-08-19T08:37:25Z | - |
dc.date.available | 2014-08-19T08:37:25Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Molecular Biology of the Cell, 20(10), 2563 - 2571, 2009 | en_US |
dc.identifier.issn | 1059-1524 | - |
dc.identifier.uri | http://www.molbiolcell.org/content/20/10/2563 | en |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/8910 | - |
dc.description | © 2009 by The American Society for Cell Biology. | en_US |
dc.description.abstract | The telomere end-protection complex prevents the ends of linear eukaryotic chromosomes from degradation or inappropriate DNA repair. The homodimeric double-stranded DNA-binding protein, Trf1, is a component of this complex and is essential for mouse embryonic development. To define the requirement for Trf1 in somatic cells, we deleted Trf1 in chicken DT40 cells by gene targeting. Trf1-deficient cells proliferated as rapidly as control cells and showed telomeric localization of Trf2, Rap1, and Pot1. Telomeric G-strand overhang lengths were increased in late-passage Trf1-deficient cells, although telomere lengths were unaffected by Trf1 deficiency, as determined by denaturing Southern and quantitative FISH analysis. Although we observed some clonal variation in terminal telomere fragment lengths, this did not correlate with cellular Trf1 levels. Trf1 was not required for telomere seeding, indicating that de novo telomere formation can proceed without Trf1. The Pin2 isoform and a novel exon 4, 5–deleted isoform localized to telomeres in Trf1-deficient cells. Trf1-deficient cells were sensitive to DNA damage induced by ionizing radiation. Our data demonstrate that chicken DT40 B cells do not require Trf1 for functional telomere structure and suggest that Trf1 may have additional, nontelomeric roles involved in maintaining genome stability. | en_US |
dc.description.sponsorship | Science Foundation Ireland, the Medical Research Council, the Cambridge European Trust, a Trinity Hall Bursary, a Sackler Award, and a Robert Gardiner Memorial Award. | en_US |
dc.language | English | - |
dc.language.iso | en | en_US |
dc.publisher | The American Society for Cell Biology | en_US |
dc.subject | Trf1 | en_US |
dc.subject | Chicken DT40 cells | en_US |
dc.subject | Telomere | en_US |
dc.subject | Gernome stability | en_US |
dc.title | Trf1 is not required for proliferation or functional telomere maintenance in chicken DT40 cells | en_US |
dc.type | Article | en_US |
dc.identifier.doi | http://dx.doi.org/10.1091/mbc.E08-10-1019 | - |
pubs.organisational-data | /Brunel | - |
pubs.organisational-data | /Brunel/Brunel Staff by College/Department/Division | - |
pubs.organisational-data | /Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences | - |
pubs.organisational-data | /Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences/Dept of Life Sciences | - |
pubs.organisational-data | /Brunel/Brunel Staff by College/Department/Division/College of Health and Life Sciences/Dept of Life Sciences/Biological Sciences | - |
pubs.organisational-data | /Brunel/Brunel Staff by Institute/Theme | - |
pubs.organisational-data | /Brunel/Brunel Staff by Institute/Theme/Institute of Environmental, Health and Societies | - |
pubs.organisational-data | /Brunel/Brunel Staff by Institute/Theme/Institute of Environmental, Health and Societies/Biomedical Engineering and Healthcare Technologies | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Brunel Institute for Ageing Studies | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Centre for Systems and Synthetic Biology | - |
Appears in Collections: | Biological Sciences Dept of Life Sciences Research Papers |
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