Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/9038
Title: Surface plasmon resonance imaging detection of silver nanoparticle-tagged immunoglobulin
Authors: Paul, S
Paul, D
Fern, GR
Ray, AK
Keywords: Plasmonics;Antibody microarray;Nanoparticle labelling
Issue Date: 2011
Publisher: The Royal Society
Citation: Journal of the Royal Society Interface, 8(61), 1204 - 1211, 2011
Abstract: The detection sensitivity of silver nanoparticle (AgNP)-tagged goat immunoglobulin G (gIgG) microarrays was investigated by studying surface plasmon resonance (SPR) images captured in the visible wavelength range with the help of a Kretchmann-configured optical coupling set-up. The functionalization of anti-gIgG molecules on the AgNP surface was studied using transmission electron microscopy, photon correlation measurements and UV–visible absorption spectroscopy. A value of 1.3 × 107 M−1 was obtained for the antibody–antigen binding constant by monitoring the binding events at a particular resonance wavelength. The detection limit of this SPR imaging instrument is 6.66 nM of gIgG achieved through signal enhancement by a factor of larger than 4 owing to nanoparticle tagging with the antibody.
Description: This article is available open access through the publisher’s website at the link below. Copyright @ 2011 The Royal Society.
URI: http://rsif.royalsocietypublishing.org/content/8/61/1204
http://bura.brunel.ac.uk/handle/2438/9038
DOI: http://dx.doi.org/10.1098/rsif.2010.0747
ISSN: 1742-5689
Appears in Collections:Wolfson Centre for Materials Processing
Dept of Mechanical Aerospace and Civil Engineering Research Papers

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