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Title: Repo-Man/PP1 regulates heterochromatin formation in interphase
Authors: Vagnarelli, P
Castro, I
Budzak, J
Di Giacinto, ML
Ligammari, L
Gokhan, E
Spanos, C
Moralli, D
Richardson, C
de las Heras, J
Salatino, S
Shirmer, E
Ullman, K
Bickmore, W
Rappsilber, J
Lamble, S
Golberg, M
Vinciotti, V
Issue Date: 2016
Publisher: Nature Publishing Group
Citation: Nature Communications, (2016)
Abstract: Repo-Man is a Protein Phosphatase 1 (PP1) targeting subunit that regulates mitotic progression and chromatin remodelling. After mitosis, Repo-Man/PP1 remains associated with chromatin but its function in interphase is not known. Here we show that Repo-Man, via Nup153, is enriched on condensed chromatin at the nuclear periphery and at the edge of the nucleopore basket. Repo-Man/PP1 regulates the formation of heterochromatin, de-phosphorylates H3S28 and it is necessary and sufficient for HP1 binding and H3K27me3 recruitment. Using a novel proteo-genomic approach, we show that Repo-Man is enriched at sub-telomeric regions together with H2AZ and H3.3 and that depletion of Repo-Man alters the peripheral localisation of a subset of these regions and alleviates repression of some polycomb telomeric genes. This study shows a role for a mitotic phosphatase in the regulation of the epigenetic landscape and gene expression in interphase.
ISSN: 2041-1723
Appears in Collections:Dept of Mathematics Research Papers

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