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http://bura.brunel.ac.uk/handle/2438/13546
Title: | Repo-Man/PP1 regulates heterochromatin formation in interphase |
Authors: | de Castro, IJ Budzak, J Di Giacinto, ML Ligammari, L Gokhan, E Spanos, C Moralli, D Richardson, C de las Heras, J Salatino, S Shirmer, E Ullman, K Bickmore, W Rappsilber, J Lamble, S Golberg, M Vinciotti, V Vagnarelli, P |
Keywords: | Cell division;Chromatin;Epigenetics |
Issue Date: | 16-Jan-2017 |
Publisher: | Nature Publishing Group |
Citation: | de Castro, I., Budzak, J., Di Giacinto, M. et al. Repo-Man/PP1 regulates heterochromatin formation in interphase. Nat Commun 8, 14048 |
Abstract: | Repo-Man is a protein phosphatase 1 (PP1) targeting subunit that regulates mitotic progression and chromatin remodelling. After mitosis, Repo-Man/PP1 remains associated with chromatin but its function in interphase is not known. Here we show that Repo-Man, via Nup153, is enriched on condensed chromatin at the nuclear periphery and at the edge of the nucleopore basket. Repo-Man/PP1 regulates the formation of heterochromatin, dephosphorylates H3S28 and it is necessary and sufficient for heterochromatin protein 1 binding and H3K27me3 recruitment. Using a novel proteogenomic approach, we show that Repo-Man is enriched at subtelomeric regions together with H2AZ and H3.3 and that depletion of Repo-Man alters the peripheral localization of a subset of these regions and alleviates repression of some polycomb telomeric genes. This study shows a role for a mitotic phosphatase in the regulation of the epigenetic landscape and gene expression in interphase. |
URI: | https://bura.brunel.ac.uk/handle/2438/13546 |
DOI: | https://doi.org/10.1038/ncomms14048 |
ISSN: | 2041-1723 |
Appears in Collections: | Dept of Mathematics Research Papers |
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