Please use this identifier to cite or link to this item:
Title: Surfactant protein D regulates murine testicular immune milieu and sperm functions
Authors: Rokade, S
Kishore, U
Madan, T
Keywords: SP-D;Testis;Sperm motility;LPS;Inflammation;Capacitation;Fertility
Issue Date: 2017
Publisher: Wiley
Citation: American Journal of Reproductive Immunology, pp. 1-57, (2017)
Abstract: Testicular levels of cytokines and immunoregulatory molecules were evaluated in lipopolysaccharide (LPS) challenged SP-D gene knock-out mice (SP-D-/-29 ). Further, sperm functions were assessed by CASA and in vitro capacitation. The effect of a recombinant fragment of human SP-D (rhSP-D) on LPS induced testicular inflammation and sperm motility was assessed in wild type (WT) mice. Result: Endogenous absence of SP-D led to significantly increased testicular levels of immunosuppressive molecules viz., Serpina3, IL-12p40, TGF-β1 and IL-10, and reduced levels of immune cell activation markers, CD86, IL-2 and ITGAX. These compensatory mechanisms resulted in markedly blunted levels of TNF-α, IL-12p40, MIP-1α, G-CSF and IL-6 in response to LPS challenge. Notably, exogenous supplementation of rhSP-D salvaged the WT mice from LPS-induced pro-inflammatory immune response and impairment of sperm motility by up39 regulating the levels of TGF-β1 and IL-10. Conclusion: The study highlights the involvement of SP-D in maintenance of testicular immune privilege and its indirect contribution to male fertility.
ISSN: 1046-7408
Appears in Collections:Dept of Life Sciences Embargoed Research Papers

Files in This Item:
File Description SizeFormat 
FullText.pdf1.4 MBAdobe PDFView/Open

Items in BURA are protected by copyright, with all rights reserved, unless otherwise indicated.