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http://bura.brunel.ac.uk/handle/2438/13763
Title: | Surfactant protein D regulates murine testicular immune milieu and sperm functions |
Authors: | Rokade, S Kishore, U Madan, T |
Keywords: | SP-D;Testis;Sperm motility;LPS;Inflammation;Capacitation;Fertility |
Issue Date: | 2017 |
Publisher: | Wiley |
Citation: | American Journal of Reproductive Immunology, pp. 1-57, (2017) |
Abstract: | Testicular levels of cytokines and immunoregulatory molecules were evaluated in lipopolysaccharide (LPS) challenged SP-D gene knock-out mice (SP-D-/-29 ). Further, sperm functions were assessed by CASA and in vitro capacitation. The effect of a recombinant fragment of human SP-D (rhSP-D) on LPS induced testicular inflammation and sperm motility was assessed in wild type (WT) mice. Result: Endogenous absence of SP-D led to significantly increased testicular levels of immunosuppressive molecules viz., Serpina3, IL-12p40, TGF-β1 and IL-10, and reduced levels of immune cell activation markers, CD86, IL-2 and ITGAX. These compensatory mechanisms resulted in markedly blunted levels of TNF-α, IL-12p40, MIP-1α, G-CSF and IL-6 in response to LPS challenge. Notably, exogenous supplementation of rhSP-D salvaged the WT mice from LPS-induced pro-inflammatory immune response and impairment of sperm motility by up39 regulating the levels of TGF-β1 and IL-10. Conclusion: The study highlights the involvement of SP-D in maintenance of testicular immune privilege and its indirect contribution to male fertility. |
URI: | http://bura.brunel.ac.uk/handle/2438/13763 |
ISSN: | 1046-7408 |
Appears in Collections: | Dept of Life Sciences Embargoed Research Papers |
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