Please use this identifier to cite or link to this item:
http://bura.brunel.ac.uk/handle/2438/16968
Title: | Cyclic-di-GMP regulates lipopolysaccharide modification and contributes to Pseudomonas aeruginosa immune evasion |
Authors: | McCarthy, RR Mazon-Moya, MJ Moscoso, JA Hao, Y Lam, JS Bordi, C Mostowy, S Filloux, A |
Issue Date: | 6-Mar-2017 |
Publisher: | Nature Research |
Citation: | McCarthy, R., Mazon-Moya, M., Moscoso, J. et al. Cyclic-di-GMP regulates lipopolysaccharide modification and contributes to Pseudomonas aeruginosa immune evasion. Nat Microbiol 2, 17027 |
Abstract: | Pseudomonas aeruginosa is a Gram-negative bacterial pathogen associated with acute and chronic infections. The universal c-di-GMP second messenger is instrumental in the switch from a motile lifestyle to resilient biofilm as in the cystic fibrosis lung. The SadC diguanylate cyclase is associated with this patho-adaptive transition. Here we identified an unrecognized SadC partner, WarA, which we show is a methyltransferase in complex with a putative kinase WarB. We established that WarA binds to c-di-GMP, which potentiates its methyltransferase activity. Together, WarA and WarB have structural similarities with the bi-functional Escherichia coli LPS O antigen regulator WbdD. Strikingly, WarA influences P. aeruginosa O antigen modal distribution and interacts with the LPS biogenesis machinery. LPS is known to modulate the immune response in the host, and by using a zebrafish infection model, we implicate WarA in the ability of P. aeruginosa to evade detection by the host. |
URI: | http://bura.brunel.ac.uk/handle/2438/16968 |
DOI: | http://dx.doi.org/10.1038/nmicrobiol.2017.27 |
ISSN: | 2058-5276 |
Appears in Collections: | Dept of Life Sciences Research Papers |
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