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Title: | An Invertebrate Burn Wound Model That Recapitulates the Hallmarks of Burn Trauma and Infection Seen in Mammalian Models |
Authors: | Evgenia, M Shi, Y Sjöberg, F Azevedo, HS David, W McCarthy, RR |
Keywords: | burn;infection;Galleria mellonella;Pseudomonas aeruginosa;MRSA;Acinetobacter baumannii;Biofilm |
Issue Date: | 3-Jun-2020 |
Publisher: | Frontiers Media |
Citation: | Maslova E. et al. (2020) 'An Invertebrate Burn Wound Model That Recapitulates the Hallmarks of Burn Trauma and Infection Seen in Mammalian Models', Frontiers in Microbiology, 11, 998, pp. 1 - 8. doi: 10.3389/fmicb.2020.00998. |
Abstract: | Copyright © 2020 Maslova, Shi, Sjöberg, Azevedo, Wareham and McCarthy. The primary reason for skin graft failure and the mortality of burn wound patients, particularly those in burn intensive care centers, is bacterial infection. Several animal models exist to study burn wound pathogens. The most commonly used model is the mouse, which can be used to study virulence determinants and pathogenicity of a wide range of clinically relevant burn wound pathogens. However, animal models of burn wound pathogenicity are governed by strict ethical guidelines and hindered by high levels of animal suffering and the high level of training that is required to achieve consistent reproducible results. In this study, we describe for the first time an invertebrate model of burn trauma and concomitant wound infection. We demonstrate that this model recapitulates many of the hallmarks of burn trauma and wound infection seen in mammalian models and in human patients. We outline how this model can be used to discriminate between high and low pathogenicity strains of two of the most common burn wound colonizers Pseudomonas aeruginosa and Staphylococcus aureus, and multi-drug resistant Acinetobacter baumannii. This model is less ethically challenging than traditional vertebrate burn wound models and has the capacity to enable experiments such as high throughput screening of both anti-infective compounds and genetic mutant libraries. |
Description: | Data Availability Statement: The datasets generated for this study are available on request to the corresponding author. |
URI: | https://bura.brunel.ac.uk/handle/2438/20724 |
DOI: | https://doi.org/10.3389/fmicb.2020.00998 |
Other Identifiers: | ORCID iD: Ronan R. McCarthy https://orcid.org/0000-0002-7480-6352 998 |
Appears in Collections: | Dept of Life Sciences Research Papers |
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FullText.pdf | Copyright © 2020 Maslova, Shi, Sjöberg, Azevedo, Wareham and McCarthy. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | 1.25 MB | Adobe PDF | View/Open |
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