Please use this identifier to cite or link to this item:
Title: Laguncularia racemosa phenolics profiling by three-phase solvent system step-gradient using high-performance countercurrent chromatography with off-line electrospray mass-spectrometry detection
Authors: Costa, FdN
Jerz, G
Hewitson, P
Figueiredo, FdS
Ignatova, S
Keywords: high-performance countercurrent chromatography;off-line MS/MS detection;three-phase solvent system;step-gradient;Laguncularia racemosa
Issue Date: 15-Apr-2021
Publisher: MDPI AG
Citation: Costa, F.d.N., Jerz, G., Hewitson, P., Figueiredo, F.d.S. and Ignatova, S. (2021) ‘Laguncularia racemosa Phenolics Profiling by Three-Phase Solvent System Step-Gradient Using High-Performance Countercurrent Chromatography with Off-Line Electrospray Mass-Spectrometry Detection’, Molecules. MDPI AG, 26 (8), 2284, pp. 1-17. doi: 10.3390/molecules26082284.
Abstract: Copyright: © 2021 by the authors. The detailed metabolite profiling of Laguncularia racemosa was accomplished by high-performance countercurrent chromatography (HPCCC) using the three-phase system n-hexane– tert-butyl methyl ether–acetonitrile–water 2:3:3:2 (v/v/v/v) in step-gradient elution mode. The gradient elution was adjusted to the chemical complexity of the L. racemosa ethyl acetate partition and strongly improved the polarity range of chromatography. The three-phase solvent system was chosen for the gradient to avoid equilibrium problems when changing mobile phase compositions encountered between the gradient steps. The tentative recognition of metabolites including the identification of novel ones was possible due to the off-line injection of fractions to electrospray ionization mass spectrometry (ESI-MS/MS) in the sequence of recovery. The off-line hyphenation profiling experiment of HPCCC and ESI-MS projected the preparative elution by selected single ion traces in the negative ionization mode. Co-elution effects were monitored and MS/MS fragmentation data of more than 100 substances were used for structural characterization and identification. The metabolite profile in the L. racemosa extract comprised flavonoids, hydrolysable tannins, condensed tannins and low molecular weight polyphenols.
Other Identifiers: 2284
Appears in Collections:Chemistry

Files in This Item:
File Description SizeFormat 
FullText.pdf3.25 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons