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Title: | Pro-apoptotic and immunotherapeutic effects of carbon nanotubes functionalized with recombinant human surfactant protein d on leukemic cells |
Authors: | Khan, HA Kishore, U Alsulami, HM Alrokayan, SH |
Keywords: | carbon nanotubes;human SP-D;cancer cells;apoptosis;immunotherapy |
Issue Date: | 28-Sep-2021 |
Publisher: | MDPI AG |
Citation: | Khan, H.A., Kishore, U., Alsulami, H.M. and Alrokayan, S.H. (2021) ‘Pro-Apoptotic and Immunotherapeutic Effects of Carbon Nanotubes Functionalized with Recombinant Human Surfactant Protein D on Leukemic Cells’, International Journal of Molecular Sciences, 22 (19), 10445, pp. 1 - 15. doi: 10.3390/ijms221910445. |
Abstract: | Copyright: © 2021 by the authors. Nanoparticles are efficient drug delivery vehicles for targeting specific organs as well as systemic therapy for a range of diseases, including cancer. However, their interaction with the immune system offers an intriguing challenge. Due to the unique physico-chemical properties, carbon nanotubes (CNTs) are considered as nanocarriers of considerable interest in cancer diagnosis and therapy. CNTs, as a promising nanomaterial, are capable of both detecting as well as delivering drugs or small therapeutic molecules to tumour cells. In this study, we coupled a recombinant fragment of human surfactant protein D (rfhSP-D) with carboxymethyl-cellulose (CMC) CNTs (CMC-CNT, 10–20 nm diameter) for augmenting their apoptotic and immunotherapeutic properties using two leukemic cell lines. The cell viability of AML14.3D10 or K562 cancer cell lines was reduced when cultured with CMC-mwCNT-coupled-rfhSP-D (CNT + rfhSP-D) at 24 h. Increased levels of caspase 3, 7 and cleaved caspase 9 in CNT + rfhSP-D treated AML14.3D10 and K562 cells suggested an involvement of an intrinsic pathway of apoptosis. CNT + rfhSP-D treated leukemic cells also showed higher mRNA expression of p53 and cell cycle inhibitors (p21 and p27). This suggested a likely reduction in cdc2-cyclin B1, causing G2/M cell cycle arrest and p53-dependent apoptosis in AML14.3D10 cells, while p53-independent mechanisms appeared to be in operation in K562 cells. We suggest that CNT + rfhSP-D has therapeutic potential in targeting leukemic cells, irrespective of their p53 status, and thus, it is worth setting up pre-clinical trials in animal models |
Description: | Data Availability Statement: Not applicable. |
URI: | https://bura.brunel.ac.uk/handle/2438/24202 |
DOI: | https://doi.org/10.3390/ijms221910445 |
ISSN: | 1661-6596 |
Other Identifiers: | 10445 |
Appears in Collections: | Dept of Life Sciences Research Papers |
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