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Title: | Carbon nanotube-coated recombinant human surfactant protein D reduces cell viability in an ovarian cancer cell line, SKOV3, and modulates mTOR pathway and pro-inflammatory cytokine response |
Authors: | Alshaya, DS Jalal, AS Alburae, NA Aljarba, NH Murugaiah, V Kishore, U Al-Qahtani, AA |
Keywords: | carbon nanotubes;rfhSP-D;drug delivery;SKOV3;anti-tumorigenic;ovarian cancer |
Issue Date: | 7-Feb-2022 |
Publisher: | Elsevier BV on behalf of King Saud University. |
Citation: | Alshaya, D.S., Jalal, A.S., Alburae, N.A., Aljarba, N.H., Murugaiah, V., Kishore, U. and Al-Qahtani, A.A. (2022) 'Carbon nanotube-coated recombinant human surfactant protein D reduces cell viability in an ovarian cancer cell line, SKOV3, and modulates mTOR pathway and pro-inflammatory cytokine response', Journal of King Saud University - Science, 34 (3), 101851, pp. 1 - 7 (7). doi: 10.1016/j.jksus.2022.101851. |
Abstract: | Copyright © 2022 The Authors. Nanoparticles such as carbon nanotubes (CNTs) have various clinical and diagnostic applications as utilised for imaging and drug delivery. Therapeutic proteins/peptides can be loaded on CNT coronas to specifically hit immune cells in overdrive or uncontrolled malignant cells. Previously, it was reported that a recombinant version of human surfactant protein D (rfhSP-D) containing trimeric C-type lectin domains induced apoptosis in several tumour cells/cell lines, including SKOV3, which is an ovarian tumour cell line. Solid-phase rfhSP-D coated on a microtiter plate is considerably more potent in inducing apoptosis in breast tumour cells. We have immobilised highly purified, endotoxin-free rfhSP-D on CNTs and assessed its antiproliferative effect on SKOV3 cells. Biotinylated rfhSP-D-CNTs were phagocytosed by SKOV3 cells, followed by apoptosis in a time-dependent manner. Gene expression analysis revealed compromised mTOR complex as a mechanism of apoptosis. When rfhSP-D-CNTs were added to a culture system of SKOV3 cells, it produced a highly proinflammatory immune response that is likely anti-tumorigenic. Thus, rfhSP-D-CNT seems a worthwhile nanocarrier for testing in vivo using an animal model of orthotopic ovarian cancer. |
URI: | https://bura.brunel.ac.uk/handle/2438/24203 |
DOI: | https://doi.org/10.1016/j.jksus.2022.101851 |
ISSN: | 1018-3647 |
Other Identifiers: | 101851 |
Appears in Collections: | Dept of Life Sciences Research Papers |
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