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Title: Impact of environmentally relevant concentrations of Bisphenol A (BPA) on the gene expression profile in an in vitro model of the normal human ovary
Authors: Zahra, A
Kerslake, R
Kyrou, I
Randeva, HS
Sisu, C
Karteris, E
Keywords: endocrine-disrupting chemicals;EDC;Bisphenol A;BPA;ovary;ovarian cancer
Issue Date: 10-May-2022
Publisher: MDPI AG
Citation: Zahra, A., Kerslake, R., Kyrou, I., Randeva, H.S., Sisu, C. and Karteris. E. (2022) ‘Impact of Environmentally Relevant Concentrations of Bisphenol A (BPA) on the Gene Expression Profile in an In Vitro Model of the Normal Human Ovary’, International Journal of Molecular Sciences, 23(10), 5334, pp. 1 - 14. doi:10.3390/ijms23105334.
Abstract: Copyright © 2022 by the authors. Endocrine-disrupting chemicals (EDCs), including the xenoestrogen Bisphenol A (BPA), can interfere with hormonal signalling. Despite increasing reports of adverse health effects associated with exposure to EDCs, there are limited data on the effect of BPA in normal human ovaries. In this paper, we present a detailed analysis of the transcriptomic landscape in normal Human Epithelial Ovarian Cells (HOSEpiC) treated with BPA (10 and 100 nM). Gene expression profiles were determined using high-throughput RNA sequencing, followed by functional analyses using bioinformatics tools. In total, 272 and 454 differentially expressed genes (DEGs) were identified in 10 and 100 nM BPA-treated HOSEpiCs, respectively, compared to untreated controls. Biological pathways included mRNA surveillance pathways, oocyte meiosis, cellular senescence, and transcriptional misregulation in cancer. BPA exposure has a considerable impact on 10 genes: ANAPC2, AURKA, CDK1, CCNA2, CCNB1, PLK1, BUB1, KIF22, PDE3B, and CCNB3, which are also associated with progesterone-mediated oocyte maturation pathways. Future studies should further explore the effects of BPA and its metabolites in the ovaries in health and disease, making use of validated in vitro and in vivo models to generate data that will address existing knowledge gaps in basic biology, hazard characterisation, and risk assessment associated with the use of xenoestrogens such as BPA.
Description: Data Availability Statement: All data is publicly available from online repositories as indicated in the materials and methods section. Supplementary Materials: The following supporting information can be downloaded at: https: //
ISSN: 1661-6596
Other Identifiers: 5334
Appears in Collections:Dept of Life Sciences Research Papers

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