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http://bura.brunel.ac.uk/handle/2438/25422
Title: | Concurrent evaluation of cytokines improves the accuracy of antibodies against Mycobacterium tuberculosis antigens in the diagnosis of active tuberculosis |
Authors: | Jacobs, R Awoniyi, DO Baumann, R Stanley, K McAnda, S Kaempfer, S Malherbe, ST Singh, M Walzl, G Chegou, NN Walzl, G Chegou, NN Kriel, M van der Spuy, G Loxton, AG Stanley, K Malherbe, ST Kriel, B Sutherland, JS Owolabi, O Sillah, A Mendy, J Gindeh, A Donkor, S Togun, T Ota, M Crampin, AC Simukonda, F Amberbir, A Chilongo, F Houben, R Kassa, D Gebrezgeabher, A Mesfin, G Belay, Y Gebremichael, G Alemayehu, Y van der Vyver, M Amutenya, FN Nelongo, JN Monye, L Sheehama, JA Iipinge, S Mayanja-Kizza, H Namuganga, AR Muzanye, G Nsereko, M Peters, P Howe, R Mihret, A Bekele, Y Tessema, B Yamuah, L Ottenhoff, THM Geluk, A Franken, K van der Ploeg-van Schip, JJ Corstjens, PLAM Tjon Kon Fat, EM de Dood, CJ Rosenkrands, I Aagaard, C Kaufmann, SHE Esterhuyse, MM Cliff, JM Dockrell, HM |
Keywords: | tuberculosis;antigens;antibodies;cytokines;biomarkers;diagnosis |
Issue Date: | 31-Jan-2022 |
Publisher: | Elsevier Ltd. |
Citation: | Jacobs, R. et al., the AE-TBC Consortium. (2022) 'Concurrent evaluation of cytokines improves the accuracy of antibodies against Mycobacterium tuberculosis antigens in the diagnosis of active tuberculosis', Tuberculosis, 133, 102169, pp. 1 - 8. doi: 10.1016/j.tube.2022.102169 |
Abstract: | Copyright © 2022 The Authors. Background: Antibodies against mycobacterial proteins are highly specific, but lack sensitivity, whereas cytokines have been shown to be sensitive but not very specific in the diagnosis of tuberculosis (TB). We assessed combinations between antibodies and cytokines for diagnosing TB. Methods: Immuoglubulin (Ig) A and IgM antibody titres against selected mycobacterial antigens including Apa, NarL, Rv3019c, PstS1, LAM, “Kit 1” (MTP64 and Tpx)”, and “Kit 2” (MPT64, Tpx and 19 kDa) were evaluated by ELISA in plasma samples obtained from individuals under clinical suspicion for TB. Combinations between the antibody titres and previously published cytokine responses in the same participants were assessed for diagnosing active TB. Results: Antibody responses were more promising when used in combination (AUC of 0.80), when all seven antibodies were combined. When anti-“Kit 1”-IgA levels were combined with five host cytokine biomarkers, the AUC increased to 97% (92–100%) with a sensitivity of 95% (95% CI, 73–100%), and specificity of 88.5% (95% CI, 68.7–97%) achieved after leave-one-out cross validation. Conclusion: When used in combination, IgA titres measured with ELISA against multiple Mycobacterium tuberculosis antigens may be useful in the diagnosis of TB. However, diagnostic accuracy may be improved if the antibodies are used in combination with cytokines. |
Description: | Data availability statement: All the important data relevant to this study was reported in the manuscript. Any additional data will be made available upon request from the corresponding author. |
URI: | https://bura.brunel.ac.uk/handle/2438/25422 |
DOI: | https://doi.org/10.1016/j.tube.2022.102169 |
ISSN: | 1472-9792 |
Other Identifiers: | ORCiD IDs: Novel N. Chegou: https://orcid.org/0000-0002-8690-1699; Jacqeline M Cliff: https://orcid.org/0000-0002-5653-1818. 102169 |
Appears in Collections: | Dept of Life Sciences Research Papers |
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FullText.pdf | Copyright © 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/). | 3.05 MB | Adobe PDF | View/Open |
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