Please use this identifier to cite or link to this item:
http://bura.brunel.ac.uk/handle/2438/25874
Title: | Elevated Circulating Lactate Levels and Widespread Expression of Its Cognate Receptor, Hydroxycarboxylic Acid Receptor 1 (HCAR1), in Ovarian Cancer |
Authors: | Kerslake, R Panfilov, S Mustafa, N Hall, M Kyrou, I Randeva, H Karteris, E Godfrey, R |
Keywords: | lactate;HCAR1;biomarker;ovarian cancer (OvCa);high-grade serous ovarian cancer (HGSOC);liquid biopsy;screening |
Issue Date: | 27-Dec-2022 |
Publisher: | MDPI AG |
Citation: | Kerslake, R. et al. (2022) 'Elevated Circulating Lactate Levels and Widespread Expression of Its Cognate Receptor, Hydroxycarboxylic Acid Receptor 1 (HCAR1), in Ovarian Cancer', Journal of Clinical Medicine, 12 (1), 217, pp. 1 - 13. doi: 10.3390/jcm12010217. |
Abstract: | Background: Augmented glycolysis in cancer cells is a process required for growth and development. The Warburg effect provides evidence of increased glycolysis and lactic acid fermentation in cancer cells. The lactate end-product of glycolysis is receiving growing traction for its role as a cell signalling molecule. Ovarian cancer (OvCa) is also characterised by altered glucose metabolism. We aim to explore circulating lactate levels in patients with high-grade serous OvCa (HGSOC) and to elucidate the expression of the lactate receptor hydroxycarboxylic acid receptor 1 (HCAR1) in OvCa. Methods: HCAR1 expression was detected in patient biopsy cores using immunohistochemistry, while lactate was measured from whole blood with a Biosen-C line clinic measuring system. Results: We noted significantly elevated lactate levels in OvCa patients (4.3 ± 1.9 mmol/L) compared with healthy controls (1.4 ± 0.6 mmol/L; p < 0.0001), with an AUC of 0.96. The HCAR1 gene is overexpressed in OvCa compared to healthy controls (p < 0.001). Using an OvCa tissue microarray (>75% expression in 100 patients), high protein expression was also recorded across all epithelial OvCa subtypes and ovarian normal adjacent tissue (NAT). Conclusions: Lactate monitoring is a simple, cost-efficient test that can offer point-of-care results. Our data suggest that the potential of circulating lactate as a screening biomarker in OvCa merits further research attention. |
Description: | Data Availability Statement: Upon request from principal investigator. Supplementary Materials: The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/jcm12010217/s1, Figure S1: Gene expression of HCAR1 (hydroxycarboxylic acid receptor 1, HCAR1; formerly known as GPR81) from The Cancer Genome Atlas (TCGA) in cancer and normal tissues. Figure S2: Bland–Altman analysis of immunohistochemistry quantification scores. Table S1: Details of tissue microarray. |
URI: | https://bura.brunel.ac.uk/handle/2438/25874 |
DOI: | https://doi.org/10.3390/jcm12010217 |
Other Identifiers: | ORCiD: Marcia Hall https://orcid.org/0000-0003-0039-5041 ORCiD: Ioannis Kyrou https://orcid.org/0000-0002-6997-3439 ORCiD: Emmanouil Karteris https://orcid.org/0000-0003-3231-7267 ORCiD: Richard Godfrey https://orcid.org/0000-0003-3700-3603 217 |
Appears in Collections: | Dept of Life Sciences Research Papers Brunel Medical School Research Papers |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
FullText.pdf | Copyright © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | 1.51 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License