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http://bura.brunel.ac.uk/handle/2438/29202| Title: | Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition) |
| Authors: | Cossarizza, A Chang, HD Radbruch, A Acs, A Adam, D Adam-Klages, S Agace, WW Aghaeepour, N Akdis, M Allez, M Almeida, LN Alvisi, G Anderson, G Andrä, I Annunziato, F Anselmo, A Bacher, P Baldari, CT Bari, S Barnaba, V Barros-Martins, J Battistini, L Bauer, W Baumgart, S Baumgarth, N Baumjohann, D Baying, B Bebawy, M Becher, B Beisker, W Benes, V Beyaert, R Blanco, A Boardman, DA Bogdan, C Borger, JG Borsellino, G Boulais, PE Bradford, JA Brenner, D Brinkman, RR Brooks, AES Busch, DH Büscher, M Bushnell, TP Calzetti, F Cameron, G Cammarata, I Cao, X Cardell, SL Casola, S Cassatella, MA Cavani, A Celada, A Chatenoud, L Chattopadhyay, PK Chow, S Christakou, E Čičin-Šain, L Clerici, M Colombo, FS Cook, L Cooke, A Cooper, AM Corbett, AJ Cosma, A Cosmi, L Coulie, PG Cumano, A Cvetkovic, L Dang, VD Dang-Heine, C Davey, MS Davies, D De Biasi, S Del Zotto, G Dela Cruz, GV Delacher, M Della Bella, S Dellabona, P Deniz, G Dessing, M Di Santo, JP Diefenbach, A Dieli, F Dolf, A Dörner, T Dress, RJ Dudziak, D Dustin, M Dutertre, CA Ebner, F Eckle, SBG Edinger, M Eede, P Ehrhardt, GRA Eich, M Engel, P Engelhardt, B Erdei, A |
| Issue Date: | 1-Oct-2019 |
| Publisher: | Wiley-VCH |
| Citation: | Cossarizza, A. et al. (2019) 'Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)', European Journal of Immunology, 49 (10), pp. 1457 - 1973. doi: 10.1002/eji.201970107. |
| Abstract: | These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion. |
| Description: | Note: In order to make the guidelines as beneficial as possible to the scientific community, if you wish to refer to a specific section when referring to these guidelines, please ensure to use the Chapter and Section information. For example: …as noted in section VII.11 of [1] … 1. Cossarizza, A., Chang, H. D., Radbruch, A., Acs, A., Adam, A., Adam-Klages, S., Agace, W. et al., Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). Eur. J. Immunol. 2019. 49: 1457–1973. The final version of the second edition published by WIley-VCH is freely available online at: https://doi.org/10.1002/eji.201970107 The third edition was published online at https://doi.org/10.1002/eji.202170126 on 7 December 2021 under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| URI: | https://bura.brunel.ac.uk/handle/2438/29202 |
| DOI: | https://doi.org/10.1002/eji.201970107 |
| ISSN: | 0014-2980 |
| Other Identifiers: | ORCiD: Cristiano Scottá https://orcid.org/0000-0003-3942-5201 |
| Appears in Collections: | Dept of Life Sciences Research Papers |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| FullText.pdf | Copyright © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Cossarizza, A. et al. (2019) 'Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)', European Journal of Immunology, 49 (10), pp. 1457 - 1973, which has been published in final form at https://doi.org/10.1002/eji.201970107. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions (see: https://authorservices.wiley.com/author-resources/Journal-Authors/licensing/self-archiving.html). | 72.63 MB | Adobe PDF | View/Open |
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