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Title: Repo-Man coordinates chromosomal reorganization with nuclear envelope reassembly during mitotic exit
Authors: Vagnarelli, P
Ribeiro, S
Sennels, L
Sanchez-Pulido, L
de Lima Alves, F
Verheyen, T
Kelly, DA
Ponting, CP
Rappsilber, J
Earnshaw, WC
Keywords: Repo-Man;Mitotic exit;Chromosomal reorganization;Nuclear envelope reassembly
Issue Date: 2011
Publisher: Elsevier
Citation: Developmental Cell, 21(2), 328 - 342, 2011
Abstract: Repo-Man targets protein phosphatase 1 γ (PP1γ) to chromatin at anaphase onset and regulates chromosome structure during mitotic exit. Here, we show that a Repo-Man:PP1 complex forms in anaphase following dephosphorylation of Repo-Man. Upon activation, the complex localizes to chromosomes and causes the dephosphorylation of histone H3 (Thr3, Ser10, and Ser28). In anaphase, Repo-Man has both catalytic and structural functions that are mediated by two separate domains. A C-terminal domain localizes Repo-Man to bulk chromatin in early anaphase. There, it targets PP1 for the dephosphorylation of histone H3 and possibly other chromosomal substrates. An N-terminal domain localizes Repo-Man to the chromosome periphery later in anaphase. There, it is responsible for the recruitment of nuclear components such as Importin β and Nup153 in a PP1-independent manner. These observations identify Repo-Man as a key factor that coordinates chromatin remodeling and early events of nuclear envelope reformation during mitotic exit.
Description: This article is available open access under a Creative Commons license ( Copyright @ 2011 Elsevier Inc.
ISSN: 1534-5807
Appears in Collections:Biological Sciences
Dept of Life Sciences Research Papers

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