Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/17712
Title: Direct removal of RNA polymerase barriers to replication by accessory replicative helicases
Other Titles: Clearing transcription barriers to replication
Authors: Hawkins, M
Dimude, JU
Howard, JAL
Smith, AJ
Dillingham, MS
Savery, NJ
Rudolph, CJ
McGlynn, P
Keywords: replication;transcription;helicases;genome stability;conflicts;RNA polymerase
Issue Date: 14-Mar-2019
Publisher: Oxford University Press
Citation: Nucleic Acids Research
Abstract: Bacterial genome duplication and transcription require simultaneous access to the same DNA template. Conflicts between the replisome and transcription machinery can lead to interruption of DNA replication and loss of genome stability. Pausing, stalling and backtracking of transcribing RNA polymerases add to this problem and present barriers to replisomes. Accessory helicases promote forkmovement through nucleoprotein barriers and exist in viruses, bacteria and eukaryotes. Here, we show that stalled Escherichia coli transcription elongation complexes block reconstituted replisomes. This physiologically relevant block can be alleviated by the accessory helicase Rep or UvrD, resulting in the formation of fulllength replication products. Accessory helicase action during replication-transcription collisions therefore promotes continued replication without leaving gaps in the DNA. In contrast, DinG does not promote replisome movement through stalled transcription complexes in vitro. However, our data demonstrate that DinG operates indirectly in vivo to reduce conflicts between replication and transcription. These results suggest that Rep and UvrD helicases operate on DNA at the replication fork whereas DinG helicase acts via a different mechanism.
URI: https://bura.brunel.ac.uk/handle/2438/17712
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz170/5380499
DOI: https://doi.org/10.1093/nar/gkz170
ISSN: 0305-1048
Appears in Collections:Dept of Life Sciences Research Papers

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