Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/25534
Title: Artificial sweeteners inhibit multidrug‐resistant pathogen growth and potentiate antibiotic activity
Authors: Proctor, CR
Maslova, E
Dzalbe, S
Rudolph, CJ
McCarthy, RR
Keywords: acinetobacter baumannii;antimicrobial;artificial sweetener;biofilm
Issue Date: 22-Nov-2022
Publisher: EMBO
Citation: McCarthy, R.R., et. al. (2022), 'Artificial sweeteners inhibit multidrug-resistant pathogen growth and potentiate antibiotic activity' in EMBO Molecular Medicine. 0 (in press), pp.1 - 21. doi: 10.15252/emmm.202216397.
Abstract: Copyright © 2022 The Authors. Antimicrobial resistance is one of the most pressing concerns of our time. The human diet is rich with compounds that alter bacterial gut communities and virulence-associated behaviours, suggesting food additives may be a niche for the discovery of novel anti-virulence compounds. Here, we identify three artificial sweeteners, saccharin, cyclamate and acesulfame-K (ace-K), that have a major growth inhibitory effect on priority pathogens. We further characterise the impact of ace-K on multidrug-resistant Acinetobacter baumannii, demonstrating that it can disable virulence behaviours such as biofilm formation, motility and the ability to acquire exogenous antibiotic-resistant genes. Further analysis revealed the mechanism of growth inhibition is through bulge-mediated cell lysis and that cells can be rescued by cation supplementation. Antibiotic sensitivity assays demonstrated that at sub-lethal concentrations, ace-K can resensitise A. baumannii to last resort antibiotics, including carbapenems. Using a novel ex vivo porcine skin wound model, we show that ace-K antimicrobial activity is maintained in the wound microenvironment. Our findings demonstrate the influence of artificial sweeteners on pathogen behaviour and uncover their therapeutic potential.
Description: Disclosure and competing interests statement: Brunel University London has two patents covering the therapeutic use of artificial sweeteners and their use to potentiate antibiotic activity.
Data availability The RNA-seq datasets produced in this study (gene expression dataset series titled "Alteration of global transcription by the artifi- cial sweetener acesulfame-K in Acinetobacter baumannii AB5075") are available at the National Center for Biotechnology Information Gene Expression Omnibus public database under accession number GSE199706 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi? acc=GSE199706).
URI: https://bura.brunel.ac.uk/handle/2438/25534
DOI: https://doi.org/10.15252/emmm.202216397
ISSN: 1757-4676
Appears in Collections:Dept of Life Sciences Research Papers

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