Please use this identifier to cite or link to this item:
Title: Artificial sweeteners inhibit multidrug‐resistant pathogen growth and potentiate antibiotic activity
Authors: Proctor, CR
Maslova, E
Dzalbe, S
Rudolph, CJ
McCarthy, RR
Keywords: acinetobacter baumannii;antimicrobial;artificial sweetener;biofilm
Issue Date: 22-Nov-2022
Publisher: EMBO
Citation: Proctor, C.R. et. al. (2022), 'Artificial sweeteners inhibit multidrug-resistant pathogen growth and potentiate antibiotic activity' in EMBO Molecular Medicine. 15 (1), e16397, pp.1 - 21. doi: 10.15252/emmm.202216397.
Abstract: Copyright © 2022 The Authors. Antimicrobial resistance is one of the most pressing concerns of our time. The human diet is rich with compounds that alter bacterial gut communities and virulence-associated behaviours, suggesting food additives may be a niche for the discovery of novel anti-virulence compounds. Here, we identify three artificial sweeteners, saccharin, cyclamate and acesulfame-K (ace-K), that have a major growth inhibitory effect on priority pathogens. We further characterise the impact of ace-K on multidrug-resistant Acinetobacter baumannii, demonstrating that it can disable virulence behaviours such as biofilm formation, motility and the ability to acquire exogenous antibiotic-resistant genes. Further analysis revealed the mechanism of growth inhibition is through bulge-mediated cell lysis and that cells can be rescued by cation supplementation. Antibiotic sensitivity assays demonstrated that at sub-lethal concentrations, ace-K can resensitise A. baumannii to last resort antibiotics, including carbapenems. Using a novel ex vivo porcine skin wound model, we show that ace-K antimicrobial activity is maintained in the wound microenvironment. Our findings demonstrate the influence of artificial sweeteners on pathogen behaviour and uncover their therapeutic potential.
Description: Disclosure and competing interests statement: Brunel University London has two patents covering the therapeutic use of artificial sweeteners and their use to potentiate antibiotic activity.
Data availability The RNA-seq datasets produced in this study (gene expression dataset series titled "Alteration of global transcription by the artifi- cial sweetener acesulfame-K in Acinetobacter baumannii AB5075") are available at the National Center for Biotechnology Information Gene Expression Omnibus public database under accession number GSE199706 ( acc=GSE199706).
ISSN: 1757-4676
Other Identifiers: ORCID iDs: Rubén de Dios; Christian Rudolph; Ronan R McCarthy
Appears in Collections:Dept of Life Sciences Research Papers

Files in This Item:
File Description SizeFormat 
FullText.pdfCopyright © 2022 The Authors. Published under the terms of the CC BY 4.0 license. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.3.58 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons