Please use this identifier to cite or link to this item:
http://bura.brunel.ac.uk/handle/2438/28056
Title: | Analysis of aflatoxin biomarkers in the hair of experimental animals |
Authors: | Mupunga, I van Rensburg, IJ Luthuli, N Abafe, OA Shai, LJ Katerere, DR |
Keywords: | aflatoxin;biomarkers;AFM1;AFB1;hair;UHPLC-MS/MS |
Issue Date: | 16-Aug-2021 |
Publisher: | MDPI |
Citation: | Mupunga, I. et al. (2021) 'Analysis of aflatoxin biomarkers in the hair of experimental animals', Toxins, 13 (8), 570, pp. 1 - 11. doi: 10.3390/toxins13080570. |
Abstract: | Copyright © 2021 by the authors. Analysis of body fluids and tissues of aflatoxin exposed individuals for the presence of aflatoxins and aflatoxin metabolites has emerged as a reliable indicator of exposure and metabolism of aflatoxins. However, current aflatoxin biomarkers are not appropriate for investigating the long-term effects of aflatoxin exposure. In this explorative study, we investigated the analysis of hair as a complementary or alternative matrix for the assessment of biomarkers of long-term aflatoxin exposure. Three groups of guinea pigs were orally dosed with 5 ugkg−1bw−1, 50 ugkg−1bw−1, and 100 ugkg−1bw−1 of AFB1. Urine and hair samples were collected on days 0, 1, 2, 3, 7, 30, 60, and 90 and analysed for AFB1 and AFM1 using UHPLC-MS/MS. AFB1 and AFM1 were detected in 75% and 13.6%, respectively, of the day 1 to day 7 urine samples. AFB1 was detected in hair samples collected from day 3 up to day 60. This is the first report to confirm the deposition of AFB1 in the hair of experimental animals. These findings indicate that hair analysis has the potential to provide an accurate long-term historical record of aflatoxin exposure with potentially important implications for the field of aflatoxin biomarkers. |
Description: | Data Availability Statement: Not applicable. |
URI: | https://bura.brunel.ac.uk/handle/2438/28056 |
DOI: | https://doi.org/10.3390/toxins13080570 |
Other Identifiers: | ORCID iD: Innocent Mupunga https://orcid.org/0000-0002-7616-9860 ORCID iD: Ovokeroye A. Abafe https://orcid.org/0000-0002-5672-6463 ORCID iD: Leshweni J. Shai https://orcid.org/0000-0003-4799-7437 ORCID iD: David R. Katerere https://orcid.org/0000-0002-4606-294X 570 |
Appears in Collections: | Dept of Life Sciences Research Papers |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
FullText.pdf | Copyright © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | 2.84 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License