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Title: | Intracellular nanovesicles mediate α5β1 integrin trafficking during cell migration |
Authors: | Larocque, G Moore, DJ Sittewelle, M Kuey, C Hetmanski, JHR La-Borde, PJ Wilson, BJ Clarke, NI Caswell, PT Royle, SJ |
Keywords: | cancer;migration;motility;trafficking |
Issue Date: | 21-Jul-2021 |
Publisher: | Rockefeller University Press |
Citation: | Larocque, G. et al. (2021) 'Intracellular nanovesicles mediate α5β1 integrin trafficking during cell migration', Journal of Cell Biology, 220 (10), e202009028, pp. 1 - 17 (+6 supplementary pp.). doi: 10.1083/jcb.202009028. |
Abstract: | Membrane traffic is an important regulator of cell migration through the endocytosis and recycling of cell surface receptors such as integrin heterodimers. Intracellular nanovesicles (INVs) are transport vesicles that are involved in multiple membrane trafficking steps, including the recycling pathway. The only known marker for INVs is tumor protein D54 (TPD54/TPD52L2), a member of the TPD52-like protein family. Overexpression of TPD52-like family proteins in cancer has been linked to poor prognosis and an aggressive metastatic phenotype, which suggests cell migration may be altered under these conditions. Here, we show that TPD54 directly binds membrane and associates with INVs via a conserved positively charged motif in its C terminus. We describe how other TPD52-like proteins are also associated with INVs, and we document the Rab GTPase complement of all INVs. Depletion of TPD52-like proteins inhibits cell migration and invasion, while their overexpression boosts motility. We show that inhibition of migration is likely due to altered recycling of α5β1 integrins in INVs. |
Description: | Data availability: The two software packages that are described in this paper, CellMigration (Royle, 2021) and CellShape (Royle, 2020), are freely available. All code that is specific to this paper is available at https://github.com/quantixed/p054p031. |
URI: | https://bura.brunel.ac.uk/handle/2438/28526 |
DOI: | https://doi.org/10.1083/jcb.202009028 |
ISSN: | 0021-9525 |
Other Identifiers: | ORCiD: Gabrielle Larocque https://orcid.org/0000-0001-8295-9378 ORCiD: Daniel J. Moore https://orcid.org/0000-0003-1553-0939 ORCiD: Méghane Sittewelle https://orcid.org/0000-0002-9383-6653 ORCiD: Cansu Kuey https://orcid.org/0000-0002-7992-3523 ORCiD: Joseph H.R. Hetmanski https://orcid.org/0000-0002-1493-351X ORCiD: Penelope J. La-Borde https://orcid.org/0000-0002-9499-0062 ORCiD: Beverley J. Wilson https://orcid.org/0000-0002-5425-7800 ORCiD: Nicholas I. Clarke https://orcid.org/0000-0003-3297-8604 ORCiD: Patrick T. Caswell https://orcid.org/0000-0002-2633-2324 ORCiD: Stephen J. Royle https://orcid.org/0000-0001-8927-6967 e202009028 |
Appears in Collections: | Dept of Life Sciences Research Papers |
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File | Description | Size | Format | |
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FullText.pdf | Copyright © 2021 Larocque et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). | 7.22 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License