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http://bura.brunel.ac.uk/handle/2438/29175| Title: | Isolation and freezing of human peripheral blood mononuclear cells from pregnant patients |
| Authors: | Efthymiou, A Mureanu, N Pemberton, R Tai-MacArthur, S Mastronicola, D Scottà, C Lombardi, G Nicolaides, KH Shangaris, P |
| Keywords: | cell biology;cell isolation;health sciences;clinical protocol;immunology;stem cells |
| Issue Date: | 25-Feb-2022 |
| Publisher: | Cell Press (Elsevier) |
| Citation: | Efthymiou, A. et al. (2022) 'Isolation and freezing of human peripheral blood mononuclear cells from pregnant patients', STAR Protocols, 3 (1), 101204, pp. 1 - 8. doi: 10.1016/j.xpro.2022.101204. |
| Abstract: | To analyze immune cell populations accurately, a large number of Peripheral Blood Mononuclear Cells (PBMCs) must be obtained from blood samples. Traditional manual isolation and SepMateTM isolation of PBMCs consistently yield blood-stained plasma layers and overall low numbers of CD4+ and CD8+ cells. Here, we describe an optimized protocol, using PBS with EDTA to increase PBMC yield from pregnant patients. This protocol enables analysis of CD4+, CD8+, and Regulatory T Cells and is potentially applicable to any immune cell population. For complete details on the use and execution of this protocol, please refer to the SepMateTM website https://www.stemcell.com/products/brands/SepMateTM-pbmc-isolation.html. |
| Description: | Data and code availability: The published article includes all datasets generated or analyzed during this study. |
| URI: | https://bura.brunel.ac.uk/handle/2438/29175 |
| DOI: | https://doi.org/10.1016/j.xpro.2022.101204 |
| Other Identifiers: | ORCiD: Cristiano Scottà https://orcid.org/0000-0003-3942-5201 ORCiD: Panicos Shangaris https://orcid.org/0000-0003-2750-8405 101204 |
| Appears in Collections: | Dept of Life Sciences Research Papers |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| FullText.pdf | Copyright © 2022 The Authors. Published by Cell Press. This is an open access article under a Creative Commons license (https://creativecommons.org/licenses/by/4.0/). | 1.52 MB | Adobe PDF | View/Open |
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