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Title: | MicroRNA-155 contributes to shear-resistant leukocyte adhesion to human brain endothelium in vitro |
Authors: | Cerutti, C Soblechero-Martin, P Wu, D Lopez-Ramirez, MA de Vries, H Sharrack, B Male, DK Romero, IA |
Keywords: | blood–brain barrier;cell adhesion molecules;flow shear stress;leukocyte adhesion;microRNA‑155;neuroinflammation |
Issue Date: | 31-May-2016 |
Publisher: | BioMed Central (part of Springer Nature) |
Citation: | Cerutti, C. et al. (2016) 'MicroRNA-155 contributes to shear-resistant leukocyte adhesion to human brain endothelium in vitro', Fluids and Barriers of the CNS, 2016, 13 (1), 8, pp. 1 - 7. doi: 10.1186/s12987-016-0032-3. |
Abstract: | Background: Increased leukocyte adhesion to brain endothelial cells forming the blood-brain barrier (BBB) es sprecedes extravasation into the central nervous system (CNS) in neuroinflammatory diseasuch as multiple sclerosis (MS). Previously, we reported that microRNA-155 (miR-155) is up-regulated in MS and by inflammatory cytokines in human brain endothelium, with consequent modulation of endothelial paracellular permeability. Here, we investigated the role of endothelial miR-155 in leukocyte adhesion to the human cerebral microvascular endothelial cell line, hCMEC/D3, under shear forces mimicking blood flow in vivo. Results: Using a gain- and loss-of-function approach, we show that miR-155 up-regulation increases leukocyte firm adhesion of both monocyte and T cells to hCMEC/D3 cells. Inhibition of endogenous endothelial miR-155 reduced monocytic and T cell firm adhesion to naïve and cytokines-induced human brain endothelium. Furthermore, this effect is partially associated with modulation of the endothelial cell adhesion molecules VCAM1 and ICAM1 by miR-155. Conclusions: Our results suggest that endothelial miR-155 contribute to the regulation of leukocyte adhesion at the inflamed BBB. Taken together with previous observations, brain endothelial miR-155 may constitute a potential molecular target for treatment of neuroinflammation diseases. |
Description: | Additional files are available online at: https://fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0032-3#Sec13 . |
URI: | https://bura.brunel.ac.uk/handle/2438/29530 |
DOI: | https://doi.org/10.1186/s12987-016-0032-3 |
Other Identifiers: | ORCiD: Camilla Cerutti https://orcid.org/0000-0001-9426-686X 8 |
Appears in Collections: | Dept of Life Sciences Research Papers |
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